RAPD typing of Klebsiella pneumoniae, Klebsiella oxytoca, Serratia marcescens and Pseudomonas aeruginosa isolates using standardized reagents Liesbeth.

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Presentation transcript:

RAPD typing of Klebsiella pneumoniae, Klebsiella oxytoca, Serratia marcescens and Pseudomonas aeruginosa isolates using standardized reagents Liesbeth Vogel, Gwendolyn Jones, Shirley Triep, Alex Koek, Lenie Dijkshoorn Clinical Microbiology and Infection Volume 5, Issue 5, Pages 270-276 (May 1999) DOI: 10.1111/j.1469-0691.1999.tb00140.x Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Scheme of the subculturing protocol performed with three isolates belonging to each species included in the study. , subculturing; DNA, preparation of template DNA. Clinical Microbiology and Infection 1999 5, 270-276DOI: (10.1111/j.1469-0691.1999.tb00140.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Examples of banding patterns obtained with (A) K. pneumoniae isolates using primer ERIC-1 (lanes 1–5) or Ready-To-Go primer 2 (lanes 6–10), and (B) K. oxytoca isolates using primer ERIC-1 (lanes 1–5) or Ready-To-Go primer 2 (lanes 6–10). Clinical Microbiology and Infection 1999 5, 270-276DOI: (10.1111/j.1469-0691.1999.tb00140.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 3 Examples of banding patterns obtained with S. marcescens isolates using Ready-To-Go primer 2 (lanes 1–5) or primer set ERIC-2/1026 (lanes 6–10). Clinical Microbiology and Infection 1999 5, 270-276DOI: (10.1111/j.1469-0691.1999.tb00140.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 4 Examples of banding patterns obtained with P. aeruginosa isolates using primer D-10514 (lanes 1–5) or primer D-14306 (lanes 6–10). Clinical Microbiology and Infection 1999 5, 270-276DOI: (10.1111/j.1469-0691.1999.tb00140.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions