Volume 65, Issue 5, Pages 1774-1781 (May 2004) Estrogen and progesterone regulate α, β, and γENaC subunit mRNA levels in female rat kidney  Lorraine Gambling, Susan Dunford, Catherine A. Wilson, Harry J. McArdle, Deborah L. Baines  Kidney International  Volume 65, Issue 5, Pages 1774-1781 (May 2004) DOI: 10.1111/j.1523-1755.2004.00593.x Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 1 Amiloride-sensitive epithelial sodium channel (ENaC) subunit mRNA abundance during development. The graphs show mRNA abundance (as% control) (see Methods section) for αENaC (A, D, and G), βENaC (B, E, and H), and γENaC (C, F, and I) in male (M) and female (F) rat kidney at birth (A to C), 10weeks (D to F) and 16weeks of age (G to I). The graphs represent data from ten samples. mRNA abundance of all ENaC subunits was significantly higher in female compared to male rats at 10 and 16weeks. *P < 0.05; **P < 0.01. Above each graph is a representative Northern blot of the ENaC subunit mRNA product together with the corresponding 18S ribosomal RNA bands from the same sample. Kidney International 2004 65, 1774-1781DOI: (10.1111/j.1523-1755.2004.00593.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 2 Amiloride-sensitive epithelial sodium channel (ENaC) subunit mRNA abundance after ovariectomy. The graphs show mRNA abundance (as % control) (see Methods section) for αENaC (A), βENaC (B), and γENaC (C) in male (M) and ovariectomized (OVX) female rats. The graphs represent data from six samples. There was no significant difference in mRNA abundance of the ENaC subunits in ovariectomized female compared to male rats. Above each graph is a representative Northern blot of the ENaC subunit mRNA product together with the corresponding 18S ribosomal RNA bands from the same sample. Kidney International 2004 65, 1774-1781DOI: (10.1111/j.1523-1755.2004.00593.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 3 α and β amiloride-sensitive epithelial sodium channel (ENaC) subunit mRNA abundance after treatment with female gonadal hormones. The graphs show mRNA abundance (as % control) (see Methods section) for αENaC (A) and βENaC (B) in ovariectomized female rats treated with vehicle (OVX), estrogen (O), or estrogen + progesterone (O + P) for 8hours and 24hours. The graphs represent data from six to seven samples. Treatment with estrogen significantly increased αENaC mRNA abundance at both time points. *P < 0.05. There was no significant effect of any treatment on βENaC mRNA abundance. Above each graph is a representative Northern blot of the ENaC subunit mRNA product at 24hours, together with the corresponding 18S ribosomal RNA bands from the same sample. Kidney International 2004 65, 1774-1781DOI: (10.1111/j.1523-1755.2004.00593.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 4 γ amiloride-sensitive epithelial sodium channel (ENaC) subunit mRNA abundance after treatment with female gonadal hormones. The graph shows mRNA abundance (as% control) (see Methods section) for γENaC in ovariectomized female rats treated with vehicle (OVX), estrogen (O), or estrogen + progesterone (O + P) or progesterone (P) for 8hours and 24hours. The graph represents data from three to seven samples. Treatment with progesterone significantly increased γENaC mRNA abundance compared to untreated ovariectomized animals at 24hours but did not quite reach significance at 8hours. *P < 0.05. Above the graph is a representative Northern blot of the ENaC subunit mRNA product at 24hours, together with the corresponding 18S ribosomal RNA bands from the same sample. Kidney International 2004 65, 1774-1781DOI: (10.1111/j.1523-1755.2004.00593.x) Copyright © 2004 International Society of Nephrology Terms and Conditions