Systematic analysis of host-pathogen interactions.

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Systematic analysis of host-pathogen interactions. Systematic analysis of host-pathogen interactions. Results of genome-wide KEGG reaction pair network analysis summarized as a graph of metabolic reactions for which the corresponding gene activity is changing during infection (|log2 fold change| > log2 1.5 and FDR < 0.05 for at least one time point p.i. [data from three biological replicates per time point]). Nodes represent metabolites, and edges represent reactions according to the KEGG main reaction pairs. Reaction directions are indicated according to the KEGG kgml pathway maps (the arrows do not necessarily correspond to reaction reversibility). Only metabolic paths connecting changing reactions with length of ≥3 are shown. G1P, glucose-1-phosphate; GAP, d-glyceraldehyde 3-phosphate; S7P, sedoheptulose 7-phosphate; DSHA, 4,5-9,10-diseco-3-hydroxy-5,9,17-tri-oxoandrosta-1(10),2-diene-4-oic acid. Upregulated genes had a log2 fold change > log2 1.5 and FDR < 0.05 for at least one time point, and downregulated genes had a log2 fold change <−log2 1.5 and FDR < 0.05 for at least one time point. Increased metabolites had a log2 fold change > log2 1.5 and FDR < 0.05 for at least one time point, and decreased metabolites had a log2 fold change <−log2 1.5 and FDR < 0.05 for at least one time point. Subgraphs were drawn with Cytoscape and manually classified into KEGG pathways (see Table S4 in the supplemental material). Michael Zimmermann et al. mSystems 2017; doi:10.1128/mSystems.00057-17