Evaluation of re-annotation for non-melanogaster Drosophila species.

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Evaluation of re-annotation for non-melanogaster Drosophila species. Evaluation of re-annotation for non-melanogaster Drosophila species. (A) Bayesian phylogenetic tree of nine Drosophila species. Nodes are supported by 100% posterior probabilities, and phylogenetic distance is shown as substitutions per site (ss). After (Chen et al, 2014). Standard three letter abbreviations are used (see text). (B) Cartoon of measurements of reads mapped to unannotated regions. Gene regions (fill), unannotated regions (open), RNA-seq reads (bars) mapped to gene regions (black) or unannotated regions (red) are shown. Numbers of reads (#), regions (in bps, flanked by dotted lines) with reads, and examples of calculating the ratios of unannotated reads are also shown. Percentage of reads (C, E) and regions with mapped reads (D, F) in unannotated regions before (C, D) and after (E, F) re-annotation. Green, yellow, and red denote low, median, and high percentage, which are also indicated in each cell. Expression profile tissues are shown (left) for female (red) and male (blue) samples. The reproductive (Repr.) tract includes the internal ducts and glands and the terminalia. The internal reproductive (Int. Repr.) tract does not include the terminalia. Missing tissues are shown (dash and open fill). Haiwang Yang et al. LSA 2018;1:e201800156 © 2018 Yang et al.