Tomato FISH Song-Bin Chang Suzanne Royer Lorrie Anderson Steve Stack.

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Presentation transcript:

Tomato FISH Song-Bin Chang Suzanne Royer Lorrie Anderson Steve Stack

in Sequencing the Tomato Genome Functions of FISH in Sequencing the Tomato Genome 1. Confirming the locations of BACs 2. Demonstrating the order of BACs 3. Determining the distance between BACs 4. Locating problem BACs 5. Determining the size of gaps between contigs

BAC 116C14, Slide 126, Short (p) Arm, Chromosome 9

* * * * * * Short arm Centromere Long arm Japan Spain Italy euchromatin heterochromatin AT-rich satellite DNA Mb 1 10 11 12 9 USA UK China Korea France 6 2 3 4 7 The Netherlands 5 8 17 16 10 13 11 24 26 19 20 27 India

45 BACs FISHED SO FAR 15 gave no FISH signal. 5 had too many repeated signals. 25 were successfully localized.

Number of BACs localized so far

SOL Genomics Network

SOL Genomics Network

Chromosome Research Send your next cytogenetics manuscript to There is no charge for color figures.

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