DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters Monica Muratori, Ph.D., Sara Marchiani, Ph.D.,

Slides:

Advertisements

Similar presentations

Isolation of spermatozoa with low levels of fragmented DNA with the use of flow cytometry and sorting Sofia C. Ribeiro, Ph.D., Gideon Sartorius, M.D.,

Advertisements

Akanksha Mehta, M. D. , Alexander Bolyakov, M. S. , Peter N

Male infertility: a biomarker of individual and familial cancer risk

Ashok Agarwal, Ph. D. , H. C. L. D. , Rakesh Sharma, Ph. D

Evaluation of chemiluminescence and flow cytometry as tools in assessing production of hydrogen peroxide and superoxide anion in human spermatozoa Reda.

Jinous Samavat, M. Sc. , Ilaria Natali, Ph. D

Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study Ashok Agarwal, Ph.D., Fnu Deepinder, M.D., Marcello.

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolas Garrido, Ph

Patient and cycle characteristics predicting high pregnancy rates with single-embryo transfer: an analysis of the Society for Assisted Reproductive Technology.

Infertility evaluation and treatment among women in the United States

Comparison of Sperm Motility Measurement Using SQA-V Automated Sperm Analyzer and Conventional Manual Methods R.K. Sharma, T.M. Said, A. Agarwal Fertility.

Fernando Zegers-Hochschild, M.D. Fertility and Sterility

Thyroid antibodies and gestational diabetes mellitus: a meta-analysis

Michael M. Alper, M.D. Fertility and Sterility

Semen cryopreservation for men banking for oligospermia, cancers, and other pathologies: prediction of post-thaw outcome using basal semen quality Selene.

Knowledge, attitudes, and practices regarding conception and fertility: a population- based survey among reproductive-age United States women Lisbet S.

Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success Luke Simon,

What is the optimal follicular size before triggering ovulation in intrauterine insemination cycles with clomiphene citrate or letrozole? An analysis.

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolás Garrido, Ph

Isolation of spermatozoa with low levels of fragmented DNA with the use of flow cytometry and sorting Sofia C. Ribeiro, Ph.D., Gideon Sartorius, M.D.,

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

Testosterone use in the male infertility population: prescribing patterns and effects on semen and hormonal parameters Mary K. Samplaski, M.D., Yasir.

Live birth of twins derived from zona-free oocytes

Association between socio-psycho-behavioral factors and male semen quality: systematic review and meta-analyses Ying Li, Ph.D., Hui Lin, Ph.D., Yafei.

Ahmad Majzoub, M. D. , Nicholas N. Tadros, M. D. , A

Cosmetics use and age at menopause: is there a connection?

How old is too old? Challenges faced by clinicians concerning age cutoffs for patients undergoing in vitro fertilization Robert L. Klitzman, M.D. Fertility.

Scoring human sperm morphology using Testsimplets and Diff-Quik slides

Reproductive outcomes of testicular versus ejaculated sperm for intracytoplasmic sperm injection among men with high levels of DNA fragmentation in semen:

Validation study of the SCREENIVF: an instrument to screen women or men on risk for emotional maladjustment before the start of a fertility treatment

Akanksha Mehta, M. D. , Alexander Bolyakov, M. S. , Peter N

Detection of oncogenic human papillomavirus genotypes on spermatozoa from male partners of infertile couples Rosaria Schillaci, Ph.D., Giuseppina Capra,

Six years' experience in ovum donation using vitrified oocytes: report of cumulative outcomes, impact of storage time, and development of a predictive.

Paweł Jóźków, M. D. , Ph. D. , Marek Mędraś, M. D. , Ph. D

Gadolinium radiologic contrast is a useful alternative for hysterosalpingography in patients with iodine allergy Dorette Noorhasan, M.D., Michael J.

Mieke Carine Wim Eeckhaut, Ph.D. Fertility and Sterility

Looking back: egg donors' retrospective evaluations of their motivations, expectations, and experiences during their first donation cycle Nancy J. Kenney,

Heat shock factor Y chromosome (HSFY) mRNA level predicts the presence of retrievable testicular sperm in men with nonobstructive azoospermia Peter J.

DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome Conrado Avendaño, M.S., Anahí.

Mary K. Samplaski, M. D. , Changhong Yu, M. S. , Michael W. Kattan, Ph

Sperm deoxyribonucleic acid damage in normozoospermic men is related to age and sperm progressive motility Stephanie Belloc, Pharm.D., Moncef Benkhalifa,

Male infertility: a biomarker of individual and familial cancer risk

Venous thromboembolism in relation to in vitro fertilization: an approach to determining the incidence and increase in risk in successful cycles Karin.

Age-specific probability of live birth with oocyte cryopreservation: an individual patient data meta-analysis Aylin Pelin Cil, M.D., Heejung Bang, Ph.D.,

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Cumulative newborn rates increase with the total number of transferred embryos according to an analysis of 15,792 ovum donation cycles Nicolás Garrido,

Evaluation of diagnostic testis biopsy and the repetition of testicular sperm extraction surgeries in infertility patients Alayman Hussein, M.B.B.C.H.,

Donatella Paoli, B. Sc. , Ph. D. , Mariagrazia Gallo, B. Sc. , Ph. D

Sources of heterogeneities in estimating the prevalence of endometriosis in infertile and previously fertile women Sun-Wei Guo, Ph.D., Yuedong Wang,

Sperm DNA fragmentation induced by cryopreservation: new insights and effect of a natural extract from Opuntia ficus-indica Mehrdad Meamar, Ph.D., Nassira.

Lindsay M. Mains, M. D. , Babak Vakili, M. D. , Yves Lacassie, M. D

Association, prevalence, and clearance of human papillomavirus and antisperm antibodies in infected semen samples from infertile patients Andrea Garolla,

Sperm selection by swim-up in terms of deoxyribonucleic acid fragmentation as measured by the sperm chromatin dispersion test is altered in heavy smokers

Fertility and Sterility: an evaluation

Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species Reda Mahfouz, M.D., Rakesh.

Sedigheh Borna, M.D., Azita Nasery, M.D. Fertility and Sterility

Hourly human chorionic gonadotropin secretion profiles during the peri-implantation period of successful pregnancies Pete N. Lohstroh, Ph.D, James W.

Looking at the endometrial biopsy with evidence-based medicine

Genetic evaluation procedures at sperm banks in the United States

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Marcia E. Garcez, M. Sc. , Cátia dos Santos Branco, M. Sc

In vitro sildenafil citrate use as a sperm motility stimulant

Saad Elzanaty, M.D., Ph.D., Johan Malm, M.D., Ph.D.

Are urinary bisphenol A levels in men related to semen quality and embryo development after medically assisted reproduction? Jure Knez, M.D., Roman Kranvogl,

Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization.

Brooks A. Keel, Ph.D., H.C.L.D.(A.B.B.) Fertility and Sterility

Progesterone level and progesterone/estradiol ratio on the day of hCG administration: detrimental cutoff levels and new treatment strategy Eman A. Elgindy,

Ongoing pregnancy rates in intrauterine insemination are affected by late follicular- phase progesterone levels Antonio Requena, M.D., María Cruz, Ph.D.,

Contact among families who share the same sperm donor

Tamer M. Said, M. D. , Geetha Ranga, M. D. , Ashok Agarwal, Ph. D. , H

Presentation transcript:

DNA fragmentation in brighter sperm predicts male fertility independently from age and semen parameters Monica Muratori, Ph.D., Sara Marchiani, Ph.D., Lara Tamburrino, Ph.D., Marta Cambi, Ph.D., Francesco Lotti, M.D., Ilaria Natali, Ph.D., Erminio Filimberti, Ph.D., Ivo Noci, M.D., Gianni Forti, M.D., Mario Maggi, M.D., Elisabetta Baldi, Ph.D. Fertility and Sterility Volume 104, Issue 3, Pages 582-590.e4 (September 2015) DOI: 10.1016/j.fertnstert.2015.06.005 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Contribution of brighter and dimmer fractions to total values of sperm DNA fragmentation (sDF) in fertile men and patients. Percentage of total sDF levels in (A) unmatched and (B) age- and semen parameters–matched (top) fertile men and (bottom) patients are expressed as deciles. Fertility and Sterility 2015 104, 582-590.e4DOI: (10.1016/j.fertnstert.2015.06.005) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Receiver operating characteristic curves analysis for total, brighter and dimmer sperm DNA fragmentation (sDF) in (A) unmatched and (B) age- and semen parameters–matched subjects. FPP = false positive proportion; TPP = true positive proportion. Fertility and Sterility 2015 104, 582-590.e4DOI: (10.1016/j.fertnstert.2015.06.005) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Intra-individual variability of sperm DNA fragmentation (sDF). Coefficient of variation (CV) of total sDF values are plotted against the time between the first and the second determinations. (B) Average CV values (n = 25) for total, brighter, and dimmer sDF and for all standard semen parameters as assessed over a 100-day period. Fertility and Sterility 2015 104, 582-590.e4DOI: (10.1016/j.fertnstert.2015.06.005) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Typical dot plots of TUNEL/PI: (left) negative control and (right) test sample. Horizontal marker distinguishes the two sperm populations brighter and dimmer. The vertical marker is established in the negative control sample to include >99% of the events and is then translated to the test sample. Note that dimmer sperm are 100% DNA fragmented (24). Fertility and Sterility 2015 104, 582-590.e4DOI: (10.1016/j.fertnstert.2015.06.005) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions