Characterization and Chromosomal Localization of Human Hair-Specific Keratin Genes and Comparative Expression During the Hair Growth Cycle  Paul E. Bowden,

Slides:



Advertisements
Similar presentations
The CXC Receptor 2 Is Overexpressed in Psoriatic Epidermis
Advertisements

A Unique Type I Keratin Intermediate Filament Gene Family is Abundantly Expressed in the Inner Root Sheaths of Sheep and Human Hair Follicles  C. Simon.
Epidermal Dysplasia and Abnormal Hair Follicles in Transgenic Mice Overexpressing Homeobox Gene MSX-2  Ting-Xin Jiang, Randall B. Widelitz, Ramendra K.
NF-κB Activity Is Required for Anagen Maintenance in Human Hair Follicles In Vitro  Jennifer E. Kloepper, Nancy Ernst, Karsten Krieger, Enikő Bodó, Tamás.
Expression of Frizzled Genes in Developing and Postnatal Hair Follicles  Seshamma T. Reddy, Thomas Andl, Min-Min Lu, Edward E. Morrisey, Sarah E. Millar,
Skin-Specific Expression of ank-393, a Novel Ankyrin-3 Splice Variant
Gene Expression of Mouse S100A3, a Cysteine-Rich Calcium-Binding Protein, in Developing Hair Follicle  Kenji Kizawa, Suguru Tsuchimoto, Keiko Hashimoto,
A Novel Human Type II Cytokeratin, K6hf, Specifically Expressed in the Companion Layer of the Hair Follicle  Hermelita Winter, Martina Jacobs, Michael.
Expression of Frizzled Genes in Developing and Postnatal Hair Follicles  Seshamma T. Reddy, Thomas Andl, Min-Min Lu, Edward E. Morrisey, Sarah E. Millar,
Expression patterns of the glial cell line–derived neurotrophic factor, neurturin, their cognate receptors GFRα-1, GFRα-2, and a common signal transduction.
Characterization of Mouse Frizzled-3 Expression in Hair Follicle Development and Identification of the Human Homolog in Keratinocytes  Betsy S. Hung,
BM-40(Osteonectin, SPARC) Is Expressed Both in the Epidermal and in the Dermal Compartment of Adult Human Skin  Nicholas Hunzelmann, Martin Hafner, Sabine.
Characterization of Lef-1 Promoter Segments that Facilitate Inductive Developmental Expression in Skin  Xiaoming Liu, Ryan R. Driskell, Meihui Luo, Duane.
John E. Olerud, Diane S. Chiu, Marcia L. Usui 
Declan P. Lunny, Erica Weed, Patrick M
Basement Membrane Zone Remodeling During Appendageal Development in Human Fetal Skin. The Absence of Type VII Collagen is Associated with Gelatinase-A.
Shinichi Inoue, Tadahiro Nambu, Toshiyasu Shimomura 
HKAP1.6 and hKAP1.7, Two Novel Human High Sulfur Keratin-Associated Proteins are Expressed in the Hair Follicle Cortex  Yutaka Shimomura, Noriaki Aoki,
Overexpression of Serpin Squamous Cell Carcinoma Antigens in Psoriatic Skin  Atsushi Takeda, Dousei Higuchi, Tadahito Takahashi, Masashi Ogo, Peter Baciu,
Volume 61, Issue 5, Pages (May 2002)
Wanglong Qiu, Xiaojun Li, Hongyan Tang, Alicia S. Huang, Andrey A
Bulge- and Basal Layer-Specific Expression of Fibroblast Growth Factor-13 (FHF-2) in Mouse Skin  Mitsuko Kawano, Satoshi Suzuki, Masashi Suzuki, Junko.
A Unique Type I Keratin Intermediate Filament Gene Family is Abundantly Expressed in the Inner Root Sheaths of Sheep and Human Hair Follicles  C. Simon.
The mRNA for Protease Nexin-1 is Expressed in Human Dermal Papilla Cells and its Level is Affected by Androgen  Tadashige Sonoda, Yuji Asada, Sotaro Kurata,
Paul G. Genever, Sarah J. Maxfield, Tim M. Skerry 
A Novel Mouse Gene, Sh3yl1, is Expressed in the Anagen Hair Follicle
Epididymal Cysteine-Rich Secretory Protein 1 Encoding Gene Is Expressed in Murine Hair Follicles and Downregulated in Mice Overexpressing Hoxc13  Ron.
Analysis of Mouse Keratin 6a Regulatory Sequences in Transgenic Mice Reveals Constitutive, Tissue-Specific Expression by a Keratin 6a Minigene  Donna.
Epidermal and Hair Follicle Progenitor Cells Express Melanoma-Associated Chondroitin Sulfate Proteoglycan Core Protein  Lucy Ghali, Soon-Tee Wong, Nick.
Size Polymorphisms in the Human Ultrahigh Sulfur Hair Keratin-Associated Protein 4, KAP4, Gene Family  Naoyuki Kariya, Yutaka Shimomura, Masaaki Ito 
Osteopontin Gene is Expressed in the Dermal Papilla of Pelage Follicles in a Hair- Cycle-Dependent Manner  Tian Yang, Pamela J. Jensen, Robert M. Lavker 
Expression Profiles of Tyrosine Kinases in Cultured Follicular Papilla Cells Versus Dermal Fibroblasts  Dawen Yu, Qiong Cao, Zhijun He, Tung-Tien Sun 
Localization of Plasminogen Activator Inhibitor Type 2 (PAI-2) in Hair and Nail: Implications for Terminal Differentiation  Robert M. Lavker, Barbara.
Expression of the Homeobox Gene, Barx2, in Wool Follicle Development
NF-κB Activity Is Required for Anagen Maintenance in Human Hair Follicles In Vitro  Jennifer E. Kloepper, Nancy Ernst, Karsten Krieger, Enikő Bodó, Tamás.
Localization of Rat FGF-5 Protein in Skin Macrophage-like Cells and FGF-5S Protein in Hair Follicle: Possible Involvement of twoFgf-5 Gene Products in.
Identification and Sequencing of a Putative Variant of Proopiomelanocortin in Human Epidermis and Epidermal Cells in Culture  Gong Can, Zalfa Abdel-Malek,
Identification of COL7A1 Alternative Splicing Inserting 9 Amino Acid Residues Into the Fibronectin Type III Linker Domain  Daisuke Sawamura, Maki Goto,
Isolation and Characterization of a Putative Keratin-Associated Protein Gene Expressed in Embryonic Skin of Mice  Mikiro Takaishi, Yoshimi Takata, Toshio.
The Neurofibromatosis Type 1 (Nf1) Tumor Suppressor is a Modifier of Carcinogen- Induced Pigmentation and Papilloma Formation in C57BL/6 Mice  Radhika.
Matthew A. Sleeman, James D. Watson, J. Greg Murison 
Expression of Calcium-Binding S100 Proteins A4 and A6 in Regions of the Epithelial Sac Associated with the Onset of Hair Follicle Regeneration  Mayumi.
Μ-Crystallin, Thyroid Hormone-binding Protein, is Expressed Abundantly in the Murine Inner Root Sheath Cells  Noriaki Aoki, Kaoru Ito, Masaaki Ito  Journal.
Type II Epithelial Keratin 6hf (K6hf) Is Expressed in the Companion Layer, Matrix, and Medulla in Anagen-Stage Hair Follicles  Zhiling Wang, Pauline Wong,
In Vivo Induction of Hair Growth by Dermal Cells Isolated from Hair Follicles After Extended Organ Culture  Mark Robinson, Amanda J. Reynolds, Ahmad Gharzi,
Dissociated Human Dermal Papilla Cells Induce Hair Follicle Neogenesis in Grafted Dermal–Epidermal Composites  Rajesh L. Thangapazham, Peter Klover, Ji-an.
K25 (K25irs1), K26 (K25irs2), K27 (K25irs3), and K28 (K25irs4) Represent the Type I Inner Root Sheath Keratins of the Human Hair Follicle  Lutz Langbein,
Deletion of the Cytoplasmatic Domain of BP180/Collagen XVII Causes a Phenotype with Predominant Features of Epidermolysis Bullosa Simplex  Marcel Huber,
Eric J. Stelnicki, László G. Kömüves, Angela O
Amplification and Overexpression of the EMS 1 Oncogene, a Possible Prognostic Marker, in Human Hepatocellular Carcinoma  Bao-Zhu Yuan, Xiaoling Zhou,
Towards Dissecting the Pathogenesis of Retinoid-Induced Hair Loss: All-Trans Retinoic Acid Induces Premature Hair Follicle Regression (Catagen) by Upregulation.
Sharon Grehan, Charles Allan, Elizabeth Tse, David Walker, John M
John E. Olerud, Marcia L. Usui, Deniz Seckin, Diane S. Chiu, Claire L
Keratinocytes Express the CD146 (Muc18/S-Endo) Antigen in Tissue Culture and During Inflammatory Skin Diseases1  Wolfgang Weninger, Michael Rendl, Michael.
Identification of Differentially Expressed Genes During a Wool Follicle Growth Cycle Induced by Prolactin  Nicholas W. Rufaut, Allan J. Pearson, Allan.
Characterization of New Members of the Human Type II Keratin Gene Family and a General Evaluation of the Keratin Gene Domain on Chromosome 12q13.13  Michael.
β-Endorphin as a Regulator of Human Hair Follicle Melanocyte Biology
Serpins in the Human Hair Follicle
Ramine Parsa, Annie Yang, Frank McKeon, Howard Green 
A Potential Suppressor of TGF-β Delays Catagen Progression in Hair Follicles  Yumiko Tsuji, Sumiko Denda, Tsutomu Soma, Laurel Raftery, Takashi Momoi,
Wook Lew  Journal of Investigative Dermatology 
Lisa H. Lerner, Abrar A. Qureshi, Bhaskar V. Reddy, Ethan A. Lerner 
Gli1 Protein is Expressed in Basal Cell Carcinomas, Outer Root Sheath Keratinocytes and a Subpopulation of Mesenchymal Cells in Normal Human Skin  Lucy.
On the Regulation of Hair Keratin Expression: Lessons from Studies in Pilomatricomas  Bernard Cribier, Bernard Peltre, Edouard Grosshans, Lutz Langbein,
Yasushi Hanakawa, Hong Li, Chenyan Lin, John R
Identification of Skn-1n, a Splice Variant Induced by High Calcium Concentration and Specifically Expressed in Normal Human Keratinocytes  Koji Nakajima,
Bart A. Jessen, Marjorie A. Phillips, Robert H. Rice 
Tsutomu Soma, Cord E. Dohrmann, Toshihiko Hibino, Laurel A. Raftery 
Transcriptional Activation of Endogenous Retroviral Sequences in Human Epidermal Keratinocytes by UVB Irradiation  Christine Hohenadl, Herbert Germaier,
Exon Skipping in IVD RNA Processing in Isovaleric Acidemia Caused by Point Mutations in the Coding Region of the IVD Gene  Jerry Vockley, Peter K. Rogan,
Presentation transcript:

Characterization and Chromosomal Localization of Human Hair-Specific Keratin Genes and Comparative Expression During the Hair Growth Cycle  Paul E. Bowden, Sandra D. Hainey, Gillian Parker, David O. Jones, Drazen Zimonjic, Nicholas Popescu, Malcolm B. Hodgins  Journal of Investigative Dermatology  Volume 110, Issue 2, Pages 158-164 (February 1998) DOI: 10.1046/j.1523-1747.1998.00097.x Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Gene structure of ghHb1 and ghHb6 is highly conserved. The gene structures of (a) ghHb1 and (b) ghHb6 are shown diagrammatically. There was only slight divergence in the intron sizes at the 5′ end of the gene but the introns at the 3′ end differed markedly. Exons 1–8 were identical in size but exon 9 differed between these two genes. Comparison of the 5′ noncoding sequences [(c) ghHb1 above and ghHb6 below] shows that the three promoter consensus sequences (HK1 box [Lef1], CAT box, and TATA box: bold and underlined) are highly conserved, as is the region around the initiation codon (ATG, bold) and the beginning of exon 1. Journal of Investigative Dermatology 1998 110, 158-164DOI: (10.1046/j.1523-1747.1998.00097.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Chromosomal in situ hybridization localizes ghHb6 to chromosome 12q13. The 6-kb Eco RI fragment (9E9) of cosmid clone 9–1a was labeled with biotin-dUTP and used for fluorescence in situ hybridization on chromosome preparations from normal peripheral leukocytes. The probe fluoresced red when the chromosomes were counter-stained with DAPI. The probe clearly marks both copies of chromosome 12 just below the centromere (→), the location of the type II keratin gene cluster. Journal of Investigative Dermatology 1998 110, 158-164DOI: (10.1046/j.1523-1747.1998.00097.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 ghHb1 and ghHb6 genes expressed in human hair follicles. Total RNA was isolated from freshly plucked human anagen hair follicles and reverse transcribed to obtain total hair follicle specific cDNA. The cDNA was then amplified by PCR using ghHb1, ghHb6, and hK14 specific primers. The generated fragments (707 bp for ghHb6, lane 2; 679 bp for ghHb1, lane 3; and 271 bp for hK14, lane 5) were analyzed by agarose gel electrophoresis. Genomic DNA contamination was ruled out by amplifying over intron 8 that increases the size of the product from gDNA (1259 bp, lane 4).174 DNA digested with Hae III was used as a standard (std;lanes 1 and 6). Journal of Investigative Dermatology 1998 110, 158-164DOI: (10.1046/j.1523-1747.1998.00097.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Human anagen hair follicles express hHa1 in keratogenous zone. Bright field autoradiographs showing sites of probe hybridization as black silver grains on a counter-stained background (hematoxylin and eosin). (a) Longitudinal section of a large anagen follicle with an anti-sense probe to hHa1 demonstrates labeling of hair cortical cells (keratogenous zone) but no labeling in the germinative matrix and cells adjacent to the dermal papilla (arrowhead). (b) In comparison, labeling with a hK14 probe shows labeling of the outer root sheath but not the germinative matrix (arrowhead) or the keratogenous zone. (c) Transverse section close to the apex of the dermal papilla (small arrowhead) showing hHa1 expression in cortical cells (arrows). (d) Transverse sections distal from the dermal papilla showing hHa1 expression throughout the cortex but absent from the central medulla (small arrowhead). (e) Transverse section at the upper limit of the keratogenous zone showing hHa1 expression restricted to the outer rim of the cortex (arrowhead). (f) Transverse section of an anagen follicle treated with a sense probe to hHa1 (negative control). (g) Longitudinal section treated with the hHa1 sense probe (negative control). The black deposits are due to melanin deposition (small arrowhead). Journal of Investigative Dermatology 1998 110, 158-164DOI: (10.1046/j.1523-1747.1998.00097.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 No hair-specific keratin expression in telogen hair follicles. (a) Longitudinal section of a telogen hair follicle shows no hHa1 hybridization in any part of the hair follicle or the adjacent sebaceous gland. (b) The same telogen hair follicle hybridized to a hK14 anti-sense probe showing a strong signal in cells of the epithelial sac surrounding the keratinized hair root (small arrowhead). The hK14 signal appears weaker in the basal cells (arrows). Journal of Investigative Dermatology 1998 110, 158-164DOI: (10.1046/j.1523-1747.1998.00097.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Isolated hair follicles culturedin vitrohave limited expression of hair-specific keratins. (a) An anti-sense probe shows expression of hHa1 in a human anagen hair follicle after 24 h in culture. The cortex is well labeled but the cell layers (small arrowhead) adjacent to the dermal papilla are not. (b) After 48 h in culture, labeling of the germinative matrix shows signs of regression away from the dermal papilla apex (arrowheads). (c) At 6 d, the cultured hair follicle shows very little hHa1 expression. The germinative matrix has regressed (arrowhead) and a prominent wrinkled glassy membrane has formed around the outer root sheath (small arrowhead). (d) After 48 h in culture, the hair follicles show hK14 expression in the lower germinative matrix (arrowhead) adjacent to the dermal papilla. Journal of Investigative Dermatology 1998 110, 158-164DOI: (10.1046/j.1523-1747.1998.00097.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions