Laurent Bozec, Marianne Odlyha Biophysical Journal

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Presentation transcript:

Thermal Denaturation Studies of Collagen by Microthermal Analysis and Atomic Force Microscopy Laurent Bozec, Marianne Odlyha Biophysical Journal Volume 101, Issue 1, Pages 228-236 (July 2011) DOI: 10.1016/j.bpj.2011.04.033 Copyright © 2011 Biophysical Society Terms and Conditions

Figure 1 Atomic force microscopy deflection images of (a) native collagen fibrils from rat tails' tendon on glass slide displaying a 67-nm banding periodicity, (b) gelatinized collagen fibrils prepared on glass slide, and (c) 38-nm banding fibrillar periodicity observed on gelatinized collagen sample. Biophysical Journal 2011 101, 228-236DOI: (10.1016/j.bpj.2011.04.033) Copyright © 2011 Biophysical Society Terms and Conditions

Figure 2 Localized thermomechanical analysis (L-TMA) curves recorded on hydrated collagen fibrils, displayed as sensor (probe deflection) versus program temperature (probe temperature). Onset of transition occurs at 58°C and the main denaturation transition occurs at 65°C. Biophysical Journal 2011 101, 228-236DOI: (10.1016/j.bpj.2011.04.033) Copyright © 2011 Biophysical Society Terms and Conditions

Figure 3 L-TMA curves recorded on dry collagen fibrils (solid line) and dry gelatin (dotted line) displayed as sensor (probe deflection) versus program temperature (probe temperature). The transitions Tcoll and Tgel correspond, respectively, to the thermal events occurring in the dry collagen and the dry gelatin. Biophysical Journal 2011 101, 228-236DOI: (10.1016/j.bpj.2011.04.033) Copyright © 2011 Biophysical Society Terms and Conditions

Figure 4 (a and d) Oblong probe imprints (star) left on the sample after performing an L-TMA with temperature ramp stopped, respectively, at 500°C (a) and 300°C (d). (b) Intact collagen matrix at a distance of 15 μm away from the border of the imprint, (c) denatured collagen matrix at the vicinity of the imprint, and (e) intact collagen matrix adjacent to the probe imprint. Biophysical Journal 2011 101, 228-236DOI: (10.1016/j.bpj.2011.04.033) Copyright © 2011 Biophysical Society Terms and Conditions

Figure 5 (a) Three-dimensional AFM image of a typical resorption pit used (5 μm deep) for the L-TMA measurement. (b) Presence of intact collagen matrix displaying a clear banding periodicity pattern. (c) L-TMA performed in the resorption lacunae. Biophysical Journal 2011 101, 228-236DOI: (10.1016/j.bpj.2011.04.033) Copyright © 2011 Biophysical Society Terms and Conditions