A simple, robust and rapid approach to detect carbapenemases in Gram-negative isolates by MALDI-TOF mass spectrometry: validation with triple quadripole.

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A simple, robust and rapid approach to detect carbapenemases in Gram-negative isolates by MALDI-TOF mass spectrometry: validation with triple quadripole tandem mass spectrometry, microarray and PCR C. Vogne, G. Prod'hom, K. Jaton, L.A. Decosterd, G. Greub Clinical Microbiology and Infection Volume 20, Issue 12, Pages O1106-O1112 (December 2014) DOI: 10.1111/1469-0691.12715 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) spectra of supernatants after 1 h incubation of a KPC Klebsiella pneumoniae positive control (red line), a negative K. pneumoniae control (blue line) and the tested strain (green line), in the presence of ertapenem. Ertapenem degradation is shown when peak A (476 Da) and peak B (498 Da) are shifted. Dotted blue lines show the position of A and B peaks for negative strains whereas the dotted red line shows the position of A and B peaks for carbapenemase-producing strains. Please note that only the B peak obtained with the Oxa48 Escherichia coli 7469 strain exhibited a clear shift. Clinical Microbiology and Infection 2014 20, O1106-O1112DOI: (10.1111/1469-0691.12715) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Ertapenem concentration as measured by MS/MS according to the presence of carbapenemase as determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Please note that the positive control obtained with the 10-mg disk in the absence of any bacterial strain is shown in the right column. Clinical Microbiology and Infection 2014 20, O1106-O1112DOI: (10.1111/1469-0691.12715) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions