Reproducibility of methylated CpG typing with the Illumina MiSeq M.L. Kampmann, O.S. Meyer, S.G Schmidt, C. Børsting, N. Morling  Forensic Science International: Genetics Supplement Series  Volume 6, Pages e430-e432 (December 2017) DOI: 10.1016/j.fsigss.2017.09.150 Copyright © 2017 Elsevier B.V. Terms and Conditions

Fig. 1 A) shows the C to T conversion at a non-CpG position as a function of the denaturation time (4–12min) for the same seven samples. Extension of the denaturation time to 12min increased the conversion efficiency of non-CpG positions without effecting the sequencing efficiency. B) Illustration of the C to T conversion at a non-CpG position as a function of the bisulfite conversion time (20–90min) for seven independent samples. After 60min, there was no further conversion of C to T. Forensic Science International: Genetics Supplement Series 2017 6, e430-e432DOI: (10.1016/j.fsigss.2017.09.150) Copyright © 2017 Elsevier B.V. Terms and Conditions

Fig. 2 Methylation percentages of 17 CpG loci in five individuals. Two buccal swabs were collected from each individual, and the DNA from each was extracted separately, followed by a separate bisulfite treatment, a separate PCR, further a separate conversion into sequencing libraries, and finally separated sequencing. Each bar therefore represents the product of one buccal swab, two PCR reactions, two bisulfite treatments, two library conversions and one sequencing performed in six replicates. The standard deviation of six replicates ranged from 1.1% to 2.42%. Forensic Science International: Genetics Supplement Series 2017 6, e430-e432DOI: (10.1016/j.fsigss.2017.09.150) Copyright © 2017 Elsevier B.V. Terms and Conditions