Conditions that countered intracellular alkalization increased M

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Conditions that countered intracellular alkalization increased M Conditions that countered intracellular alkalization increased M. smegmatis antibiotic tolerance. Conditions that countered intracellular alkalization increased M. smegmatis antibiotic tolerance. M. smegmatis was challenged with antibiotics over a pH range or in combination with DNP, CCCP, or DCCD at pH 6.6. Antibiotics were assayed in the presence of DMSO or methanol to control for vehicle effects. Antibiotics were administered at the concentrations listed in Table 3, and CFU were determined at 0 h (no pattern), 3 h (checkered), 12 h (gridded), 24 h (diagonal up), and 48 h (latticed). The data shown are means ± standard deviations from at least 3 replicates. All conditions were compared to the respective pH 6.6 time point for statistical t test analysis (*, P < 0.05; **, P < 0.01). I. L. Bartek et al. mSphere 2016; doi:10.1128/mSphere.00176-16