Biomanufacture of human platelets for transfusion: Rationale and approaches  Eun-ju Lee, Pankaj Godara, David Haylock  Experimental Hematology  Volume 42, Issue 5, Pages 332-346 (May 2014) DOI: 10.1016/j.exphem.2014.02.002 Copyright © 2014 Terms and Conditions

Figure 1 Stages of bioreactor culture and cellular differentiation required for generation of platelets from CD34+ cells. The key cytokines that drive cell differentiation and ultimately platelet production at each stage are indicated. SR1 = Stemregenin-1. Experimental Hematology 2014 42, 332-346DOI: (10.1016/j.exphem.2014.02.002) Copyright © 2014 Terms and Conditions

Figure 2 Scenarios for generating 5.0 x 1011 platelets from 5.0 x 106 CD34+ cells. The required conversion of CD34+ cells into mature MKs then into platelets is shown. A starting number of 5 x 106 CD34+ cells reflects a number easily obtained from one cord blood unit. Experimental Hematology 2014 42, 332-346DOI: (10.1016/j.exphem.2014.02.002) Copyright © 2014 Terms and Conditions