The temporal relationship between the development of vein graft intimal hyperplasia and growth factor gene expression John R. Hoch, MD, Vida K. Stark,

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Presentation transcript:

The temporal relationship between the development of vein graft intimal hyperplasia and growth factor gene expression John R. Hoch, MD, Vida K. Stark, MS, William D. Turnipseed, MD Journal of Vascular Surgery Volume 22, Issue 1, Pages 51-58 (July 1995) DOI: 10.1016/S0741-5214(95)70088-9 Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Light micrograph of native nongrafted rat epigastric vein. (Hematoxylin and eosin staining; original magnification ×250.) Journal of Vascular Surgery 1995 22, 51-58DOI: (10.1016/S0741-5214(95)70088-9) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Light micrographs of intimal hyperplasia in arterialized vein grafts. A, Section of 4-hour vein graft illustrates neutrophil adhesion to luminal surface with loss of endothelial cells. (Hematoxylin and eosin staining; original magnification × 400.) B, Section of 1 week vein graft illustrates transmural inflammatory infiltrate. (Hematoxylin and eosin staining; original magnification × 250.) C, Section of 2-week vein graft illustrating well-defined, hypercellular neointima. (Hematoxylin and eosin staining; original magnification × 400.) L represents lumen; I represents intima; N represents neointima; M represents media; A represents adventitia; arrow indicates internal elastic lamina. Journal of Vascular Surgery 1995 22, 51-58DOI: (10.1016/S0741-5214(95)70088-9) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Light micrographs of intimal hyperplasia in arterialized vein grafts. A, Section of 4-hour vein graft illustrates neutrophil adhesion to luminal surface with loss of endothelial cells. (Hematoxylin and eosin staining; original magnification × 400.) B, Section of 1 week vein graft illustrates transmural inflammatory infiltrate. (Hematoxylin and eosin staining; original magnification × 250.) C, Section of 2-week vein graft illustrating well-defined, hypercellular neointima. (Hematoxylin and eosin staining; original magnification × 400.) L represents lumen; I represents intima; N represents neointima; M represents media; A represents adventitia; arrow indicates internal elastic lamina. Journal of Vascular Surgery 1995 22, 51-58DOI: (10.1016/S0741-5214(95)70088-9) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Light micrographs of intimal hyperplasia in arterialized vein grafts. A, Section of 4-hour vein graft illustrates neutrophil adhesion to luminal surface with loss of endothelial cells. (Hematoxylin and eosin staining; original magnification × 400.) B, Section of 1 week vein graft illustrates transmural inflammatory infiltrate. (Hematoxylin and eosin staining; original magnification × 250.) C, Section of 2-week vein graft illustrating well-defined, hypercellular neointima. (Hematoxylin and eosin staining; original magnification × 400.) L represents lumen; I represents intima; N represents neointima; M represents media; A represents adventitia; arrow indicates internal elastic lamina. Journal of Vascular Surgery 1995 22, 51-58DOI: (10.1016/S0741-5214(95)70088-9) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Light micrographs of arterialized vein graft sections stained with antimonocyte/macrophage antibodies. A, Immunostaining of 1-week vein graft demonstrates transmural infiltration of monocytes and macrophages (stained with ED-1 monoclonal antibody). B, Immunostaining of 2-week vein graft illustrates extravasated macrophages present in developing neointima and adventitia. Positive immunostaining appears black (arrows); note nuclei of other cells not stained with ED-1. (Original magnification × 400.) L represents graft lumen; N represents neointima; M represents media; A represents adventitia. Journal of Vascular Surgery 1995 22, 51-58DOI: (10.1016/S0741-5214(95)70088-9) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 RT-PCR analysis of amplified TGF-ß1 (top) and PDGF-A (middle) messenger RNA. Shown are value inversion scans of ethidium bromide-stained agarose gels. G3PDH was used as internal control (bottom). EV represents epigastric vein. Journal of Vascular Surgery 1995 22, 51-58DOI: (10.1016/S0741-5214(95)70088-9) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Autoradiograph of confirmatory Southern hybridization. TGF-ß1 and PDGF-A probes hybridize specifically to their respective PCR-amplified DNA products. Journal of Vascular Surgery 1995 22, 51-58DOI: (10.1016/S0741-5214(95)70088-9) Copyright © 1995 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions