The enzyme phosphatase

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Presentation transcript:

The enzyme phosphatase Paul Beaumont & Trisha Geraghty

Phosphatase Phosphatase enzymes from plants: a versatile resource for post-16 courses Meatyard, B. (1999), J. Biol. Educ., 33(2), 109-112.

The Assay

PP = phenolphthalein (product) PPP = phenolphthalein phosphate (substrate)

Preparing the enzyme extract (Part A) Running the assay (Part B)

Creating the extract

Germination

Maceration

Loading centrifuge tube

Centrifugation

Retain supernatant

Running the Assay

Running the assay

Investigations Effect of temperature Effect of pH Phosphatase levels from different species Phosphatase levels in different locations Effect of phosphate concentration

Results