Topographic and zonal distribution of tenascin in human articular cartilage from femoral heads: normal versus mild and severe osteoarthritis K Veje,

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Topographic and zonal distribution of tenascin in human articular cartilage from femoral heads: normal versus mild and severe osteoarthritis K Veje, M.D., Jacob L Hyllested-Winge, B.Sc., Keld Ostergaard, M.D., Ph.D. Osteoarthritis and Cartilage Volume 11, Issue 3, Pages 217-227 (March 2003) DOI: 10.1016/S1063-4584(02)00368-0

Fig. 1 Histology of normal articular cartilage and articular cartilage with mild and severe OA. SFH stainings. (A) Normal articular cartilage (magnification approximately ×40). (B) Articular cartilage with mild OA showing mild fibrillation at the entire surface and loss of safranin-O staining (magnification approximately ×20). (C) Articular cartilage with severe OA showing more advanced fibrillation, a fissure through the superficial zone, but no loss of safranin-O staining intensity (magnification approximately ×20). Osteoarthritis and Cartilage 2003 11, 217-227DOI: (10.1016/S1063-4584(02)00368-0)

Fig. 2 TN stainings of normal articular cartilage and articular cartilage with mild and severe OA. (A) Normal articular cartilage from the central sampling site. Only occasional positive chondrocytes are seen in the superficial zone but a discrete line of TN staining is present in the uppermost part of the superficial zone indicating that the source of TN is primarily the synovial fluid (magnification approximately ×10). (B) Normal articular cartilage containing a cluster of chondrocytes showing the cellular localization of TN in one chondrocyte (magnification approximately ×40). (C) Central articular cartilage with mild OA showing extensive staining of the fibrillated areas. Underneath are positive chondrocytes with a discrete zone of TN in the pericellular compartment superficial to the chondrocytes (magnification approximately ×10). (D) Peripheral articular cartilage from a femoral head with mild OA. Only a few chondrocytes are positive (magnification approximately ×10). (E) Articular cartilage with severe OA showing diffuse staining in the peri- and intercellular compartments and positive chondrocytes in the middle and deep zones (magnification approximately ×20). Osteoarthritis and Cartilage 2003 11, 217-227DOI: (10.1016/S1063-4584(02)00368-0)

Fig. 2 TN stainings of normal articular cartilage and articular cartilage with mild and severe OA. (A) Normal articular cartilage from the central sampling site. Only occasional positive chondrocytes are seen in the superficial zone but a discrete line of TN staining is present in the uppermost part of the superficial zone indicating that the source of TN is primarily the synovial fluid (magnification approximately ×10). (B) Normal articular cartilage containing a cluster of chondrocytes showing the cellular localization of TN in one chondrocyte (magnification approximately ×40). (C) Central articular cartilage with mild OA showing extensive staining of the fibrillated areas. Underneath are positive chondrocytes with a discrete zone of TN in the pericellular compartment superficial to the chondrocytes (magnification approximately ×10). (D) Peripheral articular cartilage from a femoral head with mild OA. Only a few chondrocytes are positive (magnification approximately ×10). (E) Articular cartilage with severe OA showing diffuse staining in the peri- and intercellular compartments and positive chondrocytes in the middle and deep zones (magnification approximately ×20). Osteoarthritis and Cartilage 2003 11, 217-227DOI: (10.1016/S1063-4584(02)00368-0)

Fig. 3 Negative control stainings with non-immune mouse immunoglobulins. (A) Normal articular cartilage. (B) Articular cartilage with mild OA. (C) Articular cartilage with severe OA. No staining is present. Magnification approximately ×10. Osteoarthritis and Cartilage 2003 11, 217-227DOI: (10.1016/S1063-4584(02)00368-0)

Fig. 4 TN staining in the superficial zone of normal cartilage and cartilage from femoral heads with mild and severe OA at the central sampling site. Values are given as medians with the upper and lower bracket representing the 75 and the 25 percentile, respectively. The expression of TN in normal articular cartilage differs significantly from articular cartilage with mild OA (Mann–Whitney rank sum test, cellular: P=0.001, pericellular: P=0.005, and intercellular: P=0.004) and severe OA (cellular: P<0.001, pericellular: P=0.001, and intercellular: P<0.001), whereas no significant difference was present when comparing articular cartilage with mild and severe OA. See Table II for denotation of scores. Osteoarthritis and Cartilage 2003 11, 217-227DOI: (10.1016/S1063-4584(02)00368-0)

Fig. 5 TN staining in the superficial zone of peripheral localized normal cartilage and cartilage with mild and severe OA. Values are given as medians with the upper and lower bracket representing the 75 and the 25 percentile, respectively. The expression of TN is significantly increased in the cellular compartment in articular cartilage with severe OA when compared with corresponding mild OA (Mann–Whitney rank sum test, P=0.019). There is no difference between normal articular cartilage and articular cartilage with mild OA. See Table II for denotation of scores. Osteoarthritis and Cartilage 2003 11, 217-227DOI: (10.1016/S1063-4584(02)00368-0)