Promoter Hypermethylation of Multiple Genes in Hydatidiform Mole and Choriocarcinoma Wei-Cheng Xue, Kelvin Y.K. Chan, Hui-Chen Feng, Pui-Man Chiu, Hextan.

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Presentation transcript:

Promoter Hypermethylation of Multiple Genes in Hydatidiform Mole and Choriocarcinoma Wei-Cheng Xue, Kelvin Y.K. Chan, Hui-Chen Feng, Pui-Man Chiu, Hextan Y.S. Ngan, Sai-Wah Tsao, Annie N.Y. Cheung The Journal of Molecular Diagnostics Volume 6, Issue 4, Pages 326-334 (November 2004) DOI: 10.1016/S1525-1578(10)60528-4 Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Representative examples of MSP findings in normal placenta (NP), hydatidiform mole (HM), and choriocarcinoma (CCA). The PCR products in the lanes marked U showed the presence of unmethylated alleles of each gene, whereas the products in the lanes marked M indicate the presence of methylated alleles. L, size marker (pBSK/MSP I DNA marker); P, positive control; N, negative control. The Journal of Molecular Diagnostics 2004 6, 326-334DOI: (10.1016/S1525-1578(10)60528-4) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Immunostaining of E-cadherin, p16, and TIMP3 in normal placenta (NP), hydatidiform mole (HM), and choriocarcinoma (CCA). All three proteins were strongly expressed in normal placentas, but significantly reduced in choriocarcinomas showing hypermethylation. In hydatidiform moles showing hypermethylation of E-cadherin (M50) or p16 (M31), expression of corresponding proteins was reduced. Expression of TIMP3 was strong in hydatidiform mole. The Journal of Molecular Diagnostics 2004 6, 326-334DOI: (10.1016/S1525-1578(10)60528-4) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Quantitative real-time RT-PCR analysis of p16 expression in regressive mole and those developed gestational trophoblastic neoplasia (GTN) as illustrated by the ratio of signals from p16 and GAPDH housekeeping gene, measured by threshold cycle value (ΔCT). Expression of p16 was significantly lower in GTN than in regressive mole. The Journal of Molecular Diagnostics 2004 6, 326-334DOI: (10.1016/S1525-1578(10)60528-4) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions