Is quantitative PCR for the pneumolysin (ply) gene useful for detection of pneumococcal lower respiratory tract infection? G. Abdeldaim, B. Herrmann,

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Is quantitative PCR for the pneumolysin (ply) gene useful for detection of pneumococcal lower respiratory tract infection? G. Abdeldaim, B. Herrmann, J. Korsgaard, P. Olcién, J. Blomberg, K. Strålin Clinical Microbiology and Infection Volume 15, Issue 6, Pages 565-570 (June 2009) DOI: 10.1111/j.1469-0691.2009.02714.x Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1. Quantitative results of ply-based PCR applied to bronchoalveolar lavage fluid in 156 patients with lower respiratory tract infection (LRTI) and 31 controls, related to the results of other diagnostic tests. The reference standard of the LRTI patients was considered positive when at least one reference test (blood culture, urinary antigen test, or culture of bronchoalveolar lavage fluid, cut-off 104 CFUs/mL) gave a positive result, and negative when no reference test gave a positive result. In the control group, the cut-off of culture was 102 CFUs/mL. Clinical Microbiology and Infection 2009 15, 565-570DOI: (10.1111/j.1469-0691.2009.02714.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions