Benjamin T. Prince, MD, Msci, Ashley L. Devonshire, MD, MPH, Kristin A

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Presentation transcript:

Regulatory T-cell populations in children are affected by age and food allergy diagnosis Benjamin T. Prince, MD, Msci, Ashley L. Devonshire, MD, MPH, Kristin A. Erickson, Jenna Bergerson, MD, Dalia Fuleihan, BA, Christine Szychlinski, APN, Robert P. Schleimer, PhD, Paul J. Bryce, PhD, Anne Marie Singh, MD Journal of Allergy and Clinical Immunology Volume 140, Issue 4, Pages 1194-1196.e16 (October 2017) DOI: 10.1016/j.jaci.2017.04.039 Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Comparison of the percentage of CD25+CD127loFoxp3 T cells in PBMCs from children with and without food allergy. A, Comparison of CD25+CD127loFoxp3+ CD4+ T-cell populations between control and food-allergic children. B, Mean fluorescence intensity (MFI) of Foxp3 in CD25+CD127loFoxp3+ cells between food-allergic and control children. C, CD25+CD127loFoxp3+ T-cell percentage in young children (<6 years) and older children (7-17 years). D and E, Linear correlation of age and CD25+CD127loFoxp3+ percentage in control and food-allergic children. Black bars represent the median.**P = .007 for control children. *P < .05, **P < .01, Mann-Whitney U test. Journal of Allergy and Clinical Immunology 2017 140, 1194-1196.e16DOI: (10.1016/j.jaci.2017.04.039) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Phenotypic characterization of tTreg cells (CD25+CD127loFoxp3+CD4+ T cells). A, The percentage of peripheral blood tTreg cells (CD25+CD127loFoxp3+ T cells) expressing CCR6 in control and food-allergic children. B, The percentage of peripheral blood tTreg cells (CD25+CD127loFoxp3+ T cells) in control and food-allergic children in young (≤6 years) and older (>6 years) control and food-allergic children who express the putative gut-homing marker CCR6. tTREG, Thymically derived Treg. Black bars represent the median. *P = .01; #P < .1. Journal of Allergy and Clinical Immunology 2017 140, 1194-1196.e16DOI: (10.1016/j.jaci.2017.04.039) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Gating strategy for Treg cells and Treg-cell subsets. A representative patient is shown. Journal of Allergy and Clinical Immunology 2017 140, 1194-1196.e16DOI: (10.1016/j.jaci.2017.04.039) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Phenotypic characterization of Treg cells (CD25+CD127loFoxp3+CD4+ T cells). A, The percentage of peripheral blood Treg cells (CD25+CD127loFoxp3+ T cells) expressing CD45RO in control and food-allergic children. B, The percentage of peripheral blood Treg cells (CD25+CD127loFoxp3+ T cells) in control and food-allergic children in young (≤6 years) and older (>6 years) control and food-allergic children who express the memory marker, CD45RO. Black bars represent the median. *P < .05. Journal of Allergy and Clinical Immunology 2017 140, 1194-1196.e16DOI: (10.1016/j.jaci.2017.04.039) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Phenotypic characterization of T effector (CD25−CD45RO+CD4+) T cells. A, The percentage of peripheral blood T effector cells in control and food-allergic children expressing CCR6. B, The percentage of peripheral blood T effector cells in control and food-allergic children in young (≤6 years) and older (>6 years) control and food-allergic children who express CCR6. Black bars represent the median. *P < .05. Journal of Allergy and Clinical Immunology 2017 140, 1194-1196.e16DOI: (10.1016/j.jaci.2017.04.039) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 IL-10 expression of Treg cells by intracellular cytokine staining. A, IL-10 was gated by FMO control. A representative patient is shown. B, %IL-10+ cells of CD25+CD127loFoxp3+ cells on stimulation with PHA in control and food-allergic children. C, IL-10 mean fluorescence intensity (MFI). FMO, Fluorescence Minus One. Black bars represent the median. Journal of Allergy and Clinical Immunology 2017 140, 1194-1196.e16DOI: (10.1016/j.jaci.2017.04.039) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions