Detection of BRAF V600E Mutation in Colorectal Cancer

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Presentation transcript:

Detection of BRAF V600E Mutation in Colorectal Cancer Susana Benlloch, Artemio Payá, Cristina Alenda, Xavier Bessa, Montserrat Andreu, Rodrigo Jover, Antoni Castells, Xavier Llor, F. Ignacio Aranda, Bartomeu Massutí The Journal of Molecular Diagnostics Volume 8, Issue 5, Pages 540-543 (November 2006) DOI: 10.2353/jmoldx.2006.060070 Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Mutation analysis of the BRAF gene. An example of sequencing electropherogram with BRAF codon V600E heterozygous (A) and wild type (B). C: Amplification plot that displays threshold cycle (Ct) as a function of well position; Ct 0 means no amplification with specific probe, circle is allele T, and square is allele A. D: Allelic discrimination plot that determines the genotype of each tumor. NTC is no template control, Allele X means homozygous allele T, Allele Y means homozygous allele A, and Both means heterozygous alleles T and A. The Journal of Molecular Diagnostics 2006 8, 540-543DOI: (10.2353/jmoldx.2006.060070) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions