Mice over-expressing salmon calcitonin have strongly attenuated osteoarthritic histopathological changes after destabilization of the medial meniscus

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Mice over-expressing salmon calcitonin have strongly attenuated osteoarthritic histopathological changes after destabilization of the medial meniscus B.C. Sondergaard, P. Catala-Lehnen, A.K. Huebner, A.-C. Bay-Jensen, T. Schinke, K. Henriksen, S. Schilling, M. Haberland, R.H. Nielsen, M. Amling, M.A. Karsdal Osteoarthritis and Cartilage Volume 20, Issue 2, Pages 136-143 (February 2012) DOI: 10.1016/j.joca.2011.11.004 Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Timeline and design of the experimental study. Osteoarthritis and Cartilage 2012 20, 136-143DOI: (10.1016/j.joca.2011.11.004) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 The anatomy of a mouse joint. Histological section of a mouse knee joint stained by Safranin’O and Fast green. Osteoarthritis and Cartilage 2012 20, 136-143DOI: (10.1016/j.joca.2011.11.004) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Generation of ApoE-sCT TG mice. (A) Schematic representation of the injected sCT construct. (B) Northern Blot expression analysis with the SV40-pA probe revealed liver-specific expression. (C) ELISA determination of sCT and endogenous mouse CT serum concentrations in TG and WT mice. Not detectable, n.d. Osteoarthritis and Cartilage 2012 20, 136-143DOI: (10.1016/j.joca.2011.11.004) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Increased bone mass in TG mice over-expressing sCT. (A) von Kossa/van Gieson-staining of non-decalcified sections from vertebral bodies in ApoE-sCT TG mice at 6 and 12months of age. Pictures were taken with a 12.5-fold magnification. (B) Quantification of the BV/TV, Tb.N., trabecular thickness (Tb.Th.) and trabecular separation (Tb.Sp.) by static histomorphometry. Bars represent Mean±SD (n=6). Osteoarthritis and Cartilage 2012 20, 136-143DOI: (10.1016/j.joca.2011.11.004) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 ApoE-sCT mice are protected against OA progression. (A) Histological knee joint sections stained using Safranin’O and Fast green. Quantification of articular cartilage erosion in WT and ApoE-sCT TG mice using, subjected to either sham or DMM at 10week of age until 17weeks of age. WT sham-operated (n=11), WT DMM (n=11), ApoE-sCT sham-operated (n=10), ApoE-sCT DMM (n=11). All four condyles were evaluated for quantification of erosion and histopathological changes (B) an established erosion index score, sum of eroded length/sum of total length from all condyle surfaces (%) and (C) the newly developed OARSI scoring system applied to describe histopathological changes in the model. The error bars are mean±95% confidence intervals. Osteoarthritis and Cartilage 2012 20, 136-143DOI: (10.1016/j.joca.2011.11.004) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions