Molecular analysis of the PArkin co-regulated gene and association with male infertility  Gabrielle R. Wilson, B.Sc.(Hons.), Marcus L.-J. Sim, B.Sc.(Hons.), Kate M. Brody, B.Sc.(Hons.), Juliet M. Taylor, Ph.D., Robert I. McLachlan, Ph.D., Moira K. O'Bryan, Ph.D., Martin B. Delatycki, Ph.D., Paul J. Lockhart, Ph.D.  Fertility and Sterility  Volume 93, Issue 7, Pages 2262-2268 (May 2010) DOI: 10.1016/j.fertnstert.2009.01.079 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Alignment of the AluJ consensus sequence (reverse complement) and NM_152410 PACRG exon 5. The predicted exon is shown in uppercase type and boxed, the flanking intronic sequence is shown in lowercase italic type, and the predicted splice acceptor/donor consensus sequences are indicated in boldface type. Fertility and Sterility 2010 93, 2262-2268DOI: (10.1016/j.fertnstert.2009.01.079) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Analysis of the structure of the PACRG transcript in human testis. Agarose gel electrophoresis of reverse-transcription polymerase chain reaction amplification of PACRG. The amplification products using primers corresponding to both NM_152410 and AF_546876 (set 1 to set 8) are shown. The final three lanes use primer sequences specific for exon 5 of NM_152410 (set 9 to set 11). The fragment sizes of the DNA molecular weight marker are indicated in basepairs. Fertility and Sterility 2010 93, 2262-2268DOI: (10.1016/j.fertnstert.2009.01.079) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions