140mic General Microbiology

Slides:



Advertisements
Similar presentations
Bacteria (and other small topics) The Good, The Bad, and The Ugly.
Advertisements

BACTERIAL STAINING.
COLONY MORPHOLOGY ON AGAR PLATE CULTURES
Observation of bacteria using staining procedures Simple staining Gram staining.
226 PHT Lab#2 Staining techniques
GRAM STAIN PROCEDURE 1. Gather all equipment and supplies Gram stain
Do Now 1.Discuss the primary function of a microscope. 2.Define resolution and justify why electron microscopes are the best tools to use when resolving.
Microscopy Compound light microscope is composed of: 1- stand 2- stage 3- substage ( condenser, diaphragm) 4- body tube (carrying lens system) 5- light.
Use and Care of Microscope, Aseptic Technique, Smears, Simple Stains
Classification of bacteria
أ. شروق الشهراني بسم الله الرحمن الرحيم 140 MICRO LAB 6: SIMPLE STAIN + N EGATIVE S TAIN.
Negative Stain and Acid Fast Stain
One way to classify bacteria is by examining its morphology. Bacteria appear in many different shapes. We will examine the three basic shapes but note.
Gram Staining Microbiology How to Make a Smear  1. Label the frosted side of your slide with your initials, the name of the organism, and the date.
The Gram Stain In the late 1800’s, Christian Gram observed that some genera of bacteria retained a dye-Iodine complex when rinsed with alcohol, while.
226pht Lab#2 Gram staining.
Lab Exercise 4: Simple Staining and Bacterial Cell Morphology.
Microbial Ecology – From Isolation to Identification
Simple Staining and Bacterial Cell Morphology
Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique.
Simple stain Rana A.Abo-Hajjras. Simple stain The simple stain can be used to determine cell shape, size, and arrangement. True to its name, the simple.
Aseptic techniques, smear preparation and simple staining Lab # 2 Bio 261, Microbiology Prof. Santos.
What is a Stain A stain is a substance that adheres to a cell, giving the cell color. The presence of color gives the cells significant contrast so are.
Lab #5. Review for Practical #1 Colony morphology (pg. 19)
Microbiology Professor Sidelsky 2007
Focusing the microscope simple staining
Classification of bacteria Mrs. Dalia Kamal Eldien MSC in Microbiology Mrs. Dalia Kamal Eldien MSC in Microbiology Lecture NO: 3.
MICROBIOLOGY LAB 5 BIDISHA PAUL 261-BH.
Lab 3 Goals and Objectives :
Class Four Microbiology Quiz  Quiz Number One – Open Response and Identification  Microscope  Aseptic techniques  Culture transfer techniques.
Classifying and identifying Microbes. Slide preparations for bacteria A. Living preparations *Hanging drop method ** see Bacteria alive in their environment.
أ. شروق الشهراني بسم الله الرحمن الرحيم 140 MICRO LAB 6: S IMPLE S TAIN + N EGATIVE S TAIN.
Gram staining Techniques. Some history Bacteria are translucent Bacteria are translucent Staining make them visible under microscope Staining make them.
Simple Stains & Gram stain
Microbial staining Lab 6 Abeer Saati. Microbial staining Since bacterial organisms are so minute, it is impossible to view the organisms without compound.
Gram Staining Method Mr. Beyer. Individual Supplies Microscope Slide Clothes Pin Innoculating Loop Paper Towels.
Classifying and identifying Microbes
“What should ______ look like?”
Lab 2: Staining Bacterial Cells
PRACTICAL MICROBIOLOGY
Bacterial Morphology Bacteria: are unicellular free living organisms without chlorophil having doth DNAand RNA.
BACTERIA: Get to know ‘em. A “G” Science Production Copyright 2001
Gram stain.
PRACTICAL GRAM STAINING
بسم الله الرحمن الرحيم 140 Micro Lab 7: Gram stain.
Classification of bacteria
PRACTICAL GRAM STAINING
THE GRAM STAIN.
Chapter 40 Staining Specimens
Faculty of Medicine and Health Sciences
Pure Culture Techniques
PRACTICAL GRAM STAINING
Practical 2 Gram staining.
Week 4W Review cultures: Exp 2B Lab Two & Exp 3 Lab Two
I cant talk, I have laryngitis
MICROSCOPIC EXAMINATION OF BACTERIA (UNSTAINED & STAINED SMEARS)
The process of adding a dye to a bacterial culture
Crystal Gram ’ s (Primary stain) Gram’s violet iodine Safranin Alcohol.
Dr. Souki MICRO 200 Cerritos College
Morphology of bacteria
Biology and Biotechnology department
طريقة تخطيط الاطباق Streak Plate Method
The Structure of Bacteria
BIOLOGY & BIOTECHNOLODY DEPARTMENT
Practical No. 5 Staining Department of Microbiology
Simple Staining and Bacterial Cell Morphology
SHAPE OF THE BACTERIA.
Bacterial Structure (Lab 3)
Simple stain.
COLONY MORPHOLOGY ON AGAR PLATE CULTURES
Presentation transcript:

140mic General Microbiology Madeha Al-Onazi

SIMPLE STAIN + NEGATIVE STAIN LAB 6 Staining SIMPLE STAIN + NEGATIVE STAIN

PURPOSE To recognize the three basic shapes of bacterial cells.

THE THREE COMMON SHAPES OF BACTERIA

1- Coccus Having one of the following arrangements:  Diplococcus: a pair of cocci  Streptococcus: a chain of cocci  Tetrad: a square of 4 cocci  Sarcina: a cube of 8 cocci  Staphylococcus: cocci in irregular, often grape-like clusters

2-Bacillus.  Bacillus: a single bacillus  Streptobacillus: bacilli in chains  Coccobacillus: oval and similar to a coccus

3-Spiral  Vibrio: an incomplete spiral or comma-shaped  Spirillum: a thick, rigid spiral  Spirochete: a thin, flexible spiral

SIMPLE STAIN

SIMPLE STAIN :  The simple stain is a very simple staining procedure involving only one stain.  You may choose from methylene blue, safranin, and crystal violet.

The Method 1. Prepare the smear. - place a small drop of water on a clean slide. Drag the sterile inoculating needle tip through the edge of colony. - Gently spread the mixture into a circle to spread out.

SIMPLE STAIN : 2. Let the smear air dry completely.

SIMPLE STAIN : 3. Heat-Fix the smear.  Smears are heat-fixed by quickly passing the slide through a flame two or three times.  This causes the microbes to stick to the slide and not get washed off during the staining process.

SIMPLE STAIN : 4. Stain the smear.  Place the slide on a rack over the sink. Flood the smear with stain and let it for 60-90 seconds. Rinse gently and blot dry.

SIMPLE STAIN : 5. Then, place a drop of oil directly on the stained smear .Turn the oil lens into position and fine focus to observe the cells.

RESULT

Coccus (cocci pl.)

Bacillus (Bacilli pl.)

Spirillum (Spirilli pl.)

NEGATIVE STAIN

NEGATIVE STAIN 1. Place a very small drop of nigrosin near one end of a well- cleaned and flamed slide. 2. Remove a small amount of the culture with an inoculating loop and disperse it in the drop of stain . 3. Use another clean slide to spread the drop of stain containing the organism using the following technique.

NEGATIVE STAIN 5. Allow the smear to dry without heating. 6. Observe the cells with microscope.

NEGATIVE STAIN

RESULT Negatively Stained Bacillus Negatively Stained Cocci