Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes A. Marsano, M.Sc., S.J. Millward-Sadler,

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General Information Osteoarthritis and Cartilage

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Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes A. Marsano, M.Sc., S.J. Millward-Sadler, Ph.D., D.M. Salter, M.D., A. Adesida, Ph.D., T. Hardingham, Ph.D., E. Tognana, Ph.D., E. Kon, M.D., C. Chiari-Grisar, M.D., S. Nehrer, M.D., M. Jakob, M.D., I. Martin, Ph.D. Osteoarthritis and Cartilage Volume 15, Issue 1, Pages 48-58 (January 2007) DOI: 10.1016/j.joca.2006.06.009 Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Proliferation index for IMC, FPC, SMC and AC expanded without control (CTR) or with growth factors (TFP). ∗=significantly different from same cells expanded without TFP. °=significantly different from IMC, FPC and SMC. Osteoarthritis and Cartilage 2007 15, 48-58DOI: (10.1016/j.joca.2006.06.009) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Representative sections of pellets generated by meniscus (A, B), FPC (C, D), SMC (E, F) and AC (G, H) expanded with (B, D, F, H) or without (A, C, E, G) the growth factor combination TFP and stained by Safranin-O. Bar=100μm. Osteoarthritis and Cartilage 2007 15, 48-58DOI: (10.1016/j.joca.2006.06.009) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Fractions of GAG (A) and mRNA expression levels of collagen types I (B), II (C) and Sox9 (D) in pellets generated by IMC, FPC, SMC and AC, expanded without (control, CTR) or with growth factors (TFP). ∗=significantly different from same cell source expanded without TFP. °=significantly different from all the other cell sources, expanded in the same condition. §=significantly different from AC, expanded in the same condition. Osteoarthritis and Cartilage 2007 15, 48-58DOI: (10.1016/j.joca.2006.06.009) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Representative Safranin-O stained sections of tissues generated by culture of meniscus (A), FPC (B), SMC (C) and AC (D) into Hyaff®-11 for 6 weeks. Bar=300μm. Osteoarthritis and Cartilage 2007 15, 48-58DOI: (10.1016/j.joca.2006.06.009) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Representative immunohistochemical stain for collagen types I (A, B), II (C, D), III (E, F), IV (G, H) and VI (I, J) of cartilaginous tissues generated by culture of AC (A, C, E, G, I) and synovial membrane cells (B, D, F, H, J) into Hyaff®-11 for 4 weeks. Immunohistochemical stains were similar in tissues generated by meniscus, FPC and SMC. Bar=100μm. Osteoarthritis and Cartilage 2007 15, 48-58DOI: (10.1016/j.joca.2006.06.009) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Representative Safranin-O stained sections of tissues generated by culture of meniscus (A), FPC (B), SMC (C) and AC (D) into Hyaff®-11 for 2 weeks, followed by 6 weeks of ectopic implantation. Bar=300μm. Osteoarthritis and Cartilage 2007 15, 48-58DOI: (10.1016/j.joca.2006.06.009) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions