The DHX15–CMTR1 interaction inhibits translation of a subset of genes.

Slides:

Advertisements

Similar presentations

RNA Quantitation from RNAseq Data

Advertisements

Additional file 8: Estimation of biological variations

p < Figure S1 p55 viral protein level Relative HIV-1

by Elena A. Federzoni, Peter J. M. Valk, Bruce E

Poly-GR and poly-PR interact with overlapping proteins in primary neurons and in HEK293 cells. Poly-GR and poly-PR interact with overlapping proteins in.

FOXP3 regulates cAMP production by regulating miR‐142‐3p and AC9.

FOG-1 represses GATA-1-dependent FcϵRI β-chain transcription: transcriptional mechanism of mast-cell-specific gene expression in mice by Keiko Maeda, Chiharu.

Loss of SMARCA4 expression or activity derepresses miR-27a.

by Thomas T. Murooka, Ramtin Rahbar, Leonidas C

HIS-24 regulates expression of infection-inducible genes.

Volume 138, Issue 5, Pages e3 (May 2010)

Volume 120, Issue 6, Pages (March 2005)

CMTR1 methyltransferase stimulates DHX15 helicase activity.

Volume 23, Issue 5, Pages (May 2018)

Analysis of total and polysomal RNA clk-1(qm30) and clk-1(qm30)+WT.

Patient neural progenitors show a neurodevelopmental phenotype.

Modification of the βIII-tubulin C-terminal tail does not affect tubulin polymerization or the cellular proliferation rate. Modification of the βIII-tubulin.

HuR physically interacts with the Il2ra 3′UTR mRNA and enhances its translational efficiency in activated CD4+ T cells. HuR physically interacts with the.

Volume 49, Issue 3, Pages (February 2013)

Volume 15, Issue 8, Pages (May 2016)

Dynamic change of transcription pausing through modulating NELF protein stability regulates granulocytic differentiation by Xiuli Liu, Aishwarya A. Gogate,

Functional Modulation of Gene Expression by Ultraconserved Long Non-coding RNA TUC338 during Growth of Human Hepatocellular Carcinoma Hui-Ju Wen, Michael.

Lea Goentoro, Marc W. Kirschner Molecular Cell

TSA dose-dependent increase in transcription.

The histone H3.3K36M mutation reprograms the epigenome of chondroblastomas by Dong Fang, Haiyun Gan, Jeong-Heon Lee, Jing Han, Zhiquan Wang, Scott M. Riester,

Characterising the CMTR1–DHX15 interaction.

Molecular Mechanisms Regulating the Defects in Fragile X Syndrome Neurons Derived from Human Pluripotent Stem Cells Tomer Halevy, Christian Czech, Nissim.

Volume 19, Issue 4, Pages (October 2003)

Volume 56, Issue 5, Pages (December 2014)

Volume 16, Issue 4, Pages (November 2004)

CMTR1 interacts with DHX15.

CMTR1 methyltransferase activity is repressed by DHX15.

Fmrp regulates E‐cadherin and Vimentin in breast cancer cells Fmrp (red) and E‐cadherin (green) detection by I.F. in 4T1 cells expressing different Fmrp.

DNMT3A variants cause unique DNAm signatures.

Alterations in mRNA 3′ UTR Isoform Abundance Accompany Gene Expression Changes in Human Huntington’s Disease Brains Lindsay Romo, Ami Ashar-Patel, Edith.

Limited transcriptomic changes upon HOTAIR RNA overexpression in MDA‐MB‐231 breast cancer cells Limited transcriptomic changes upon HOTAIR RNA overexpression.

Impact of RTEL1 variants on telomere maintenance.

Significant differences in translational efficiencies of DNA damage repair pathway genes between patient clusters. Significant differences in translational.

Volume 8, Issue 2, Pages (July 2014)

HDAC11 regulates CCL2 expression by recruiting PU.1.

Sucrose gradient analysis of ribosomes after repressing the genes for 60S r-proteins uL4 and uL22 and 60S assembly factors Rpf2 and Rrs1. Sucrose gradient.

Differential protein, mRNA, lncRNA and miRNA regulation by p53.

ACF1 loss perturbs gene expression in early embryos.

Volume 129, Issue 6, Pages (June 2007)

USP2a overexpression modifies the microRNA expression profile of prostate cells. USP2a overexpression modifies the microRNA expression profile of prostate.

Fig. 3 Conserved genomic association of PRC1 activity in different leukemic cells. Conserved genomic association of PRC1 activity in different leukemic.

Summary of RNA FISH quantifications.

TSA induces transcription from CCND1-MS2 alleles under CCND1 promoter control. TSA induces transcription from CCND1-MS2 alleles under CCND1 promoter control.

Scatter plots represent log2 (FPKM (average) +1) values for genes in control and KO/KD samples. Scatter plots represent log2 (FPKM (average) +1) values.

Oxidative stress globally impairs translation.

(A) Western blot probing nuclear extract from wild-type (wt) and the newly generated ACF1 mutant (AcfC) embryos (0–16 h). (A) Western blot probing nuclear.

CD4+CLA+CD103+ T cells from human blood and skin share a transcriptional profile. CD4+CLA+CD103+ T cells from human blood and skin share a transcriptional.

CREB1 promotes the induction of endogenous ERα target genes.

miR-145 regulates IGF1R expression.

MiRNA expression profiling in adipocytes from wild-type (WT) and leptin-deficient ob/ob mice. miRNA expression profiling in adipocytes from wild-type (WT)

mRNA adenosine-to-inosine editing increases under DR.

Analysis of the 55S peak appearing after repressing the gene for 40S r-protein uS4. Analysis of the 55S peak appearing after repressing the gene for 40S.

Reduced OXPHOS expression and increased UCP expression.

Modification of the β-tubulin C-terminal tail does not affect the partitioning of tubulin between the soluble and polymerized fractions in response to.

BCL-3 regulates expression of stemness-associated Wnt targets.

CD25 expression identifies two transcriptionally distinct subsets of very early effector cells. CD25 expression identifies two transcriptionally distinct.

Fig. 3 The rs risk enhancer is a hub for intrachromosomal and interchromosomal interactions. The rs risk enhancer is a hub for intrachromosomal.

Volume 62, Issue 3, Pages (May 2016)

EZH2 overexpression establishes a unique and conserved super-enhancer–associated transcriptional landscape. EZH2 overexpression establishes a unique and.

Secretion of TNF-α from macrophages and monocytic cells were reduced by the deletion of Nrdc. Secretion of TNF-α from macrophages and monocytic cells were.

Expression of the cell death markers activated caspase-3 and LC3 I/II in TUBO cells. Expression of the cell death markers activated caspase-3 and LC3 I/II.

FGFR1 is dominantly expressed in mesenchymal-like KRAS-mutant lung cancer cell lines. FGFR1 is dominantly expressed in mesenchymal-like KRAS-mutant lung.

Levels and activity of c-myc are increased in a series of stably transfected DAOY and UW228 medulloblastoma cell lines. Levels and activity of c-myc are.

KDM4A levels affect the distribution of translation initiation factors

Cxxc1-deficient TH17 cells exhibit a Treg cell–like expression profile

Presentation transcript:

The DHX15–CMTR1 interaction inhibits translation of a subset of genes. The DHX15–CMTR1 interaction inhibits translation of a subset of genes. (A) Extracts from HCC1806 cells transfected with pCDNA5 HA-CMTR1, 2L/A, or vector control (V) were centrifuged through 10%–50% sucrose gradients. 259 nm absorbance was determined across the gradient in four independent experiments. Representative experiment presented. The fraction of the gradients analysed by RNAseq is indicated by shaded boxes. The identities of the peaks in the polysome profiles is indicated. (B) Cell extracts were analysed by Western blot. (C) Average and standard deviation of ratio of polysome to mononosome absorbance for four independent experiments. RNAseq analysis was used to determine uniquely aligned reads per gene (transcript isoforms collapsed) in RNA and polysome samples from HCC1806 cells expressing HA-CMTR1 WT and 2L/A (n = 2). (D) Scatter plot of log2 transformed total counts per million (log2CPM) in total RNA. (E) Scatter plot of log2-fold change (FC) in 2L/A/WT for total RNA and polysomal RNA. Significantly regulated genes determined by a negative binomial rest in EdgeR in red. (F) Scatter plot of log2-transformed counts per million (log2CPM) in polysomal RNA. (G) Polysomal mRNA levels quantified by RT–PCR. Average and standard deviation for three independent experiments. t test was performed ***P-value < 0.005. (H) Western blot analysis of proteins in HCC1806 cells expressing pCDNA5 HA-CMTR1, 2L/A, or vector control (V). Charts represented average protein signal in Western blots and standard deviation for three independent experiments. Quantitation performed in ImageJ software. Francisco Inesta-Vaquera et al. LSA 2018;1:e201800092 © 2018 Inesta-Vaquera et al.