UIEF1/2 interact with UAP56 in yeast and plant cells.

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UIEF1/2 interact with UAP56 in yeast and plant cells. UIEF1/2 interact with UAP56 in yeast and plant cells. A, N-terminal amino acid sequence of UIEF1. The UBM is highlighted and the residues that were changed into Ala residues in UIEF1m are indicated by (A). B, Yeast two-hybrid assays with cells harboring the indicated constructs grown on DDO (left) or QDO (right) media. C, Protein interactions analyzed by acceptor-photobleaching FRET. N. benthamiana leaves were coinfiltrated with vectors (x axis) directing the expression of the indicated donor (eGFP, green)-acceptor (mCherry, red) combinations as well as of positive and negative controls. Transiently transformed cells were analyzed in two biological replicates by FRET-APB. Mean FRET-APB efficiencies (±sd, eight analyzed nuclei each) are shown, which were analyzed using Student’s t test (***P < 0.001; n.s.: nonsignificant). Hans F. Ehrnsberger et al. Plant Physiol. 2019;179:1525-1536 ©2019 by American Society of Plant Biologists