Detection of a Peripheral Blood T Cell Clone is an Independent Prognostic Marker in Mycosis Fungoides Elisabeth A. Fraser-Andrews, Alison J. Woolford,

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Detection of a Peripheral Blood T Cell Clone is an Independent Prognostic Marker in Mycosis Fungoides Elisabeth A. Fraser-Andrews, Alison J. Woolford, Robin Russell-Jones, Sean J. Whittaker Journal of Investigative Dermatology Volume 114, Issue 1, Pages 117-121 (January 2000) DOI: 10.1046/j.1523-1747.2000.00830.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 PCR/SSCP analysis of the TCR VγI gene family. PCR amplification was performed in this case using the VγI and JγC primers. N, non-denatured samples; D, denatured samples. Lanes 1 and 2, blood and skin, respectively, from a patient with T3 MF; lanes 3 and 4, different patients with plaque stage MF; lane 5, a patient with Sézary syndrome (incidental to this study); lane 6, Jurkat T cell line. Journal of Investigative Dermatology 2000 114, 117-121DOI: (10.1046/j.1523-1747.2000.00830.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Detection sensitivity of PCR/SSCP analysis of the TCR VγI gene family. DNA from Jurkat T cell line was serially diluted with DNA from a PBL mononuclear sample from a normal volunteer. N, nondenatured samples; D, denatured sample. Journal of Investigative Dermatology 2000 114, 117-121DOI: (10.1046/j.1523-1747.2000.00830.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Sequence analysis of DNA eluted from polyacrylamide gels using the VgII primer. →, Splice site. Samples 1 and 2, analysis of DNA eluted from a band in the non-denatured and denatured lanes, respectively, from the same patient. The sequence shows the VγII and Jγ2 sequence separated by the N region. Sample 3, analysis of DNA eluted from a smear in the denatured lane. Journal of Investigative Dermatology 2000 114, 117-121DOI: (10.1046/j.1523-1747.2000.00830.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Results of SBA and PCR/SSCP analysis on 63 samples. Journal of Investigative Dermatology 2000 114, 117-121DOI: (10.1046/j.1523-1747.2000.00830.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions