Figure 3. Structural observations

Slides:

Advertisements

Similar presentations

Human Cellular Physiology PHSI3004/3904 Secreted signals and synaptic transmission Dr Bill Phillips Dept of Physiology, Anderson Stuart Bldg Rm N348.

Advertisements

Figure 10.6 Structure of the Neuromuscular Junction Process Diagrams Step-by-Step Copyright © 2007 by John Wiley & Sons, Inc.

Synapse Formation I April 18, 2007 Mu-ming Poo 1.Steps in the formation of neuromuscular junction 2.Early events of synaptogenesis 3.ACh receptor aggregation.

Synapse formation (2) Effect of postsynaptic cell on presynaptic differentiation –Retrograde signals involved in active zone formation –Synapse elimination.

Motor Unit: A motor neuron and all the muscle fibers it innervates. *Powerful movements use more fibers *Fine movements use fewer fibers.

Special Topics in Neuroscience. Pre- and postsynaptic membranes at the neuromuscular synapse are highly specialized postjunctional folds Schwann cell.

The Neuromuscular Junction

09.12 Function of the Neuromuscular Junction Slide number 1 Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.

Postsynaptic Membrane Ion Channels ACh-R PhC a PIP 3 Dg PkC i a Endoplasmic Reticulum R NK1 c -R NK1 SP c -R NK1 -G R R ER IP 3 3 -R ER Pr PPhos + PhC.

Nerve Supply to a Muscle. Nerve Supply Motor Neuron: Nerve that stimulates a muscle cell. A single nerve (motor neuron) will supply approximately 150.

Synaptic Transmission

Disorders of Muscle Excitability

163-1 Myasthenia/Thymoma.

Analysis of the deep-sequencing data.

Subcellular distribution of presynaptic CaV2

1. An action potential arrives at the

Figure 3 Immunohistochemical analyses of positive and negative Epstein-Barr virus (EBV) control tissues using immunostaining Immunohistochemical analyses.

The Drosophila tumor suppressor gene, dlg, is involved in structural plasticity at a glutamatergic synapse Bo Guan, Beate Hartmann, Young-Ho Kho, Michael.

Figure 1. Type and distribution within KCNQ2 protein of variants in self-limiting epilepsy vs epileptic encephalopathy Type and distribution within KCNQ2.

Figure Sural nerve electron microscopy

Volume 46, Issue 4, Pages (May 2005)

1. An action potential arrives at the

Muscle Innervation Skeletal muscles are acted upon by somatic motor neurons (neurons that take thought) Motor unit: axon terminal branches of the neuron.

Figure 1 Neuromuscular junction in myasthenia gravis (MG)

Volume 111, Issue 3, Pages (November 2002)

Figure 1 Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient.

Figure 2 Vastus lateralis biopsy of P2 (A) Vastus lateralis muscle cryostat section hematoxylin and eosin (H&E) staining shows basophilic subsarcolemmal.

Volume 120, Issue 3, Pages (February 2005)

David B. Ress, Mark L. Harlow, Robert M. Marshall, Uel J. McMahan

Neuromuscular Junction

Figure 1 Dominant and recessive missense and nonsense variants in neurofilament light (NEFL)‏ Dominant and recessive missense and nonsense variants in.

Volume 31, Issue 6, Pages (September 2001)

The Drosophila tumor suppressor gene, dlg, is involved in structural plasticity at a glutamatergic synapse Bo Guan, Beate Hartmann, Young-Ho Kho, Michael.

Figure 5 Neurite structure is not disrupted by the lack of neurofilament light (NEFL)‏ Neurite structure is not disrupted by the lack of neurofilament.

Mobility of Calcium Channels in the Presynaptic Membrane

Mitofilin clusters are localized at the horizontal sides of mitochondria. Mitofilin clusters are localized at the horizontal sides of mitochondria. (A.

Figure 3 qRT-PCR fold change comparison, disease vs CON, of splice vs no-splice primer sites qRT-PCR fold change comparison, disease vs CON, of splice.

Figure 3 Transport activity of human SLC25A4 and SLC25A4 p.Lys33Gln

Figure 2 Linkage analysis of chromosome 19

Figure 2 JCV index JCV index (A) Fifty samples of natalizumab-treated patients with multiple sclerosis were assessed twice for their anti-JCV antibody.

Axon Branch Removal at Developing Synapses by Axosome Shedding

Unrestricted Synaptic Growth in spinster—a Late Endosomal Protein Implicated in TGF- β-Mediated Synaptic Growth Regulation Sean T Sweeney, Graeme W Davis

Volume 96, Issue 11, Pages (June 2009)

Figure Genetic deletion and MRI changes with EHMT1 deletion

Figure 2 Functionally significant genes

Figure 1 JCV serostatus JCV serostatus (A) Serostatus of 1,921 natalizumab-treated patients with multiple sclerosis, with JCV− patients shown in black.

Synucleins Have Multiple Effects on Presynaptic Architecture

Figure 3 Detection of synapsin Ia, Ib, and IIa in cell-based assays, colocalization of patient IgA and commercial synapsin antibodies in hippocampus sections.

Figure 5 ADP-induced inflammatory responses require P2Y12 receptors in human microgliaIncreasing concentrations of ADP (5, 50, and 200 μM) increased tumor.

Volume 20, Issue 3, Pages (March 1998)

Volume 15, Issue 5, Pages R154-R158 (March 2005)

Figure 2 Immunohistological detection of EBV latent and early lytic proteins in MS and control brains Immunohistological detection of EBV latent and early.

Figure 3 Expression of mutant STIM1 in cultured cells and its effect on store-operated Ca2+ influx Expression of mutant STIM1 in cultured cells and its.

Figure 2 SORL1 mutants' overexpression increases β-amyloid secretion

Figure 2 DNM1 mutations inhibit transferrin uptake Inhibition of transferrin internalization in mammalian cell lines. DNM1 mutations inhibit transferrin.

Figure 5 Electron microscopy of transfected HeLa cells and Dnm1Ftfl neurons (A) Electron microscopy (EM) of HeLa cells transfected with wild type (WT)

Figure 5 Multiple immunohistochemistry for various RNA-binding proteins and VCP/p97 Representative microphotographs of transverse cryosections from the.

Figure 3 ADAM22 mutant proteins do not bind to LGI1

Figure Clinical, radiologic, and histopathologic findings

Figure ND5 and MCARNE phenotype

In Vivo Time-Lapse Imaging of Synaptic Takeover Associated with Naturally Occurring Synapse Elimination Mark K. Walsh, Jeff W. Lichtman Neuron Volume.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

AChRs are distributed in stripes that are not correlated with the crest of NMJ junctional folds. AChRs are distributed in stripes that are not correlated.

Figure 1 Classical pathway and lectin pathway activity in patients with multifocal motor neuropathy and controls Classical pathway (CP) activity (A) and.

Figure 2 Cell-based assay demonstrating differential binding of AChR antibodies to the adult and fetal receptorsThe fetal (gamma subunit specific) and.

Rab18−/− mice have large accumulations of neurofilament at the neuromuscular junction. Rab18−/− mice have large accumulations of neurofilament at the neuromuscular.

Transmission & Impulses Across a Synapse

Ciliary Trafficking: CEP290 Guards a Gated Community

An ipRGC axon collateral colocalized with the presynaptic marker bassoon makes contact with TH-positive DAC processes. An ipRGC axon collateral colocalized.

Electron micrograph of large IC neuron with dense VGLUT2+ axosomatic endings. a, VGLUT2+ terminals contain an electron-dense reaction product and surround.

Presentation transcript:

Figure 3. Structural observations Structural observations (A) ATPase reaction after preincubation at pH 9.4 reveals type 2 fiber atrophy and type 1 fiber hypertrophy. (B) Cytochemical reaction for AChE in longitudinally oriented fibers shows pretzel-shaped or linearly arrayed EP regions. (C and D) Colocalization of AChE (green signal) and the AChR (red signal) in cryosections of transversely oriented fibers reveals normal expression of both proteins at the EPs. (E) Electron micrograph depicts a normal EP region. Inset in (E) shows docking of synaptic vesicles at an active zone marked by a thickened segment of the presynaptic membrane. (F) Localization of AChR with peroxidase-labeled α-bungarotoxin. The black reaction product for AChR is normally distributed on terminal expansions of the junctional folds. Bars: (A, B): 50 μm; (C, D): 25 μm; (E, F): 0.5 μm; and inset in (E): 0.1 μm. AChE = acetylcholinesterase; AChR = acetylcholine receptor; AZ = active zone; EP = endplate; JF = junctional fold. Andrew G. Engel et al. Neurol Genet 2016;2:e105 © 2016 American Academy of Neurology