Structural Biophysics, Niels Bohr Institute

Slides:

Advertisements

Similar presentations

1 Catalog 1 Check the radiation damagep. 2 2 Check aggregation by AutoRg program. Calculate theoretical I 0, MW, and V tot with protein SAXS I 0 estimtaion.xls.

Advertisements

Bayesian inference of normal distribution

Lecture : 2 Light scattering and determination of the size of macromolecules.

ME221Lecture 91 ME 221 Statics Lecture #9 Sections 9.1 – 9.6.

Exercise Exercise3.1 8 Exercise3.1 9 Exercise

Exercise Exercise Exercise Exercise

Exercise Exercise Exercise Exercise

Macromolecular Small-Angle X-ray Scattering

Bridging the solution divide: comprehensive structural analyses of dynamic RNA, DNA, and protein assemblies by small-angle X-ray scattering By Rambo and.

Exercise Exercise6.1 7 Exercise6.1 8 Exercise6.1 9.

Macromolecular Small-Angle Scattering with Synchrotron Radiation Tom Irving BioCAT, Dept. BCPS and CSRRI Illinois Institute of Technology.

Determination of grain sizes and porosity in nanophase vanadium oxide and V/Ce oxide with GISAXS and GIXR Aleksandra Turković Ruđer Bošković Institute.

STRUCTURE PECULIARITIES OF α- CRYSTALLIN STUDIED BY SMALL ANGLE NEUTRON AND X-RAY SCATTERING T.N. Murugova 1, O.I. Ivankov 1,5, A.I. Kuklin 1,3, K.O. Muranov.

Suggested Changes to the non- conservative low frequency criteria for the seismic design spectrum used in intraplate regions John Nichols Department of.

Students: Carmen Breazu, Univ. of Craiova, Romania

Shapes and Angle Rules 80 + ? = = 180.

GUIDELINES FOR STUDENTS. Investigating Correlation Think/ Explore at least 10 real life examples regarding the relationship of two variables Draw scatter.

NIELS BOHR COLE TODD AND ZACH SHEEHAN. WHAT, WHERE, WHY Niels Bohr announced the theory of electron placement in 1913, while studying at Cambridge.

Directed Numbers. SandcastleHole in the sand +1 or = = (+1) = (-1) = 0.

Determination of some features of apoferritin using small angle neutron scattering The whole list of the participants: 1. Marek Rataj JINR group where.

Autocovariance Structures for Radial Averages in Small Angle X-Ray Scattering Experiments Andreea Erciulescu, Jay Breidt, Mark van der Woerd Departments.

Static Light Scattering Part 2: Solute characteristics

Protocol: Isolation of proteins Determination of protein concentration

Shapes in real space ––> reciprocal space

Fourier Transform Infrared Spectroscopy

Scatter Plots and Association

Blot, Blot, Western Baby Kristin B. Dupre June 30th, 2011.

X AND R CHART EXAMPLE IN-CLASS EXERCISE

Volume 93, Issue 1, Pages (July 2007)

Crystallography H. K. D. H. Bhadeshia Introduction and point groups

2. Find the equation of line of regression

Indirect Fourier Transformation (IFT)

Lab Activity 5 Biuret Method

GEOMETRY Units and 13.

Water and Backbone Dynamics in a Hydrated Protein

Digital Image Processing

Indirect Fourier Transformation (IFT)

Volume 101, Issue 1, Pages (July 2011)

Lab Activity 6 Biuret Method

Refolding of a High Molecular Weight Protein: Salt Effect on Collapse

Volume 87, Issue 2, Pages (August 2004)

Protocol: Isolation of proteins Determination of protein concentration

Structure and Plasticity of Endophilin and Sorting Nexin 9

Volume 94, Issue 2, Pages (January 2008)

Volume 98, Issue 1, Pages (January 2010)

Structure and Plasticity of Endophilin and Sorting Nexin 9

Katherine Launer-Felty, C. Jason Wong, James L. Cole

Zhaoyong Xi, Matthew J. Whitley, Angela M. Gronenborn Structure

Volume 110, Issue 1, Pages (January 2016)

Triangle sum property.

2nd exam coverage X-ray sources lab rotating anode synchrotrons

The Exomer Cargo Adaptor Features a Flexible Hinge Domain

High-Pressure SAXS Study of Folded and Unfolded Ensembles of Proteins

Validating Solution Ensembles from Molecular Dynamics Simulation by Wide-Angle X- ray Scattering Data Po-chia Chen, Jochen S. Hub Biophysical Journal

C.2.10 Sample Questions.

C.2.8 Sample Questions.

C.2.8 Sample Questions.

Amyloid Fibrillation of Insulin under Water-Limited Conditions

10.3 The Inverse z-Transform

Binding of Alkyl Polyglucoside Surfactants to Bacteriorhodopsin and its Relation to Protein Stability M. Gabriella Santonicola, Abraham M. Lenhoff, Eric.

Volume 114, Issue 3, Pages (February 2018)

EE150: Signals and Systems 2016-Spring

Half-life of removal from subcutaneous administration site after subcutaneous administration of fluorescence-labeled VEGF-C156S, ovalbumin (OVA), bovine.

Kiyoshi Kawai, Toru Suzuki, Masaharu Oguni Biophysical Journal

Small-Angle X-Ray Scattering of the Cholesterol Incorporation into Human ApoA1- POPC Discoidal Particles Søren Roi Midtgaard, Martin Cramer Pedersen,

Jochen Zimmer, Declan A. Doyle, J. Günter Grossmann

Protein Structure and Hydration Probed by SANS and Osmotic Stress

Purified LF is cytotoxic to WEHI 164 cells.

Presentation transcript:

Structural Biophysics, Niels Bohr Institute Exercise on data analysis of Small-angle scattering data from Bovine Serum Albumin in solution Lise Arleth, Professor Structural Biophysics, Niels Bohr Institute University of Copenhagen, Faculty of Life Sciences 1

Non-trivial structure/shape! Bovine serum albumin M = 66 000 Da Non-trivial structure/shape! 2

Exercise: SAXS analysis of Bovine Serum Albumin (BSA) Use Svergun’s PRIMUS -Plot data -Make background subtractions -Normalize by sample concentration – what do you see? -Make Guinier plots and determine Radius of Gyration for the different concentrations – what do you see? Try to explain -Make Indirect Fourier Transform using GNOM. Discuss. What would be the next steps? Next steps: Kør dammin53qw.exe on 4 mg/ml sample and try to determine the shape.