Volume 16, Issue 1, Pages (January 2008)

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Volume 16, Issue 1, Pages 137-148 (January 2008) Vibrio cholerae Toxin-Coregulated Pilus Structure Analyzed by Hydrogen/Deuterium Exchange Mass Spectrometry  Juliana Li, Mindy S. Lim, Sheng Li, Melissa Brock, Michael E. Pique, Virgil L. Woods, Lisa Craig  Structure  Volume 16, Issue 1, Pages 137-148 (January 2008) DOI: 10.1016/j.str.2007.10.027 Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 1 Comparison of Morphology and Protease Susceptibility for TCPWT and TCPH181A Transmission electron micrographs of (A) TCPWT and (B) TCPH181A showing similar morphologies and bundling characteristics, though TCPWT bundle to a greater extent. (C) Coomassie-stained SDS-PAGE of chymotrypsin-digested TCPH181A (lanes 1, 4, and 7), TCPWT (lanes 2, 5, and 8), and the TcpA monomer (lanes 3, 6, and 9) in the absence (0) and presence (+) of chymotrypsin at a chymostrypsin:protein mass ratio of 3:1. Both pilus forms are equally resistant to chymotrypsin proteolysis, remaining intact after a 60 min digestion. In contrast, monomeric TcpA was rapidly degraded at an early time point. Structure 2008 16, 137-148DOI: (10.1016/j.str.2007.10.027) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 2 Overlapping Peptides Identified by Pepsin Digest and LC-MS Peptide maps of (A) the TCP filament and (B) the TcpA monomer. Amino acids are numbered according to the mature TcpA sequence. Structure 2008 16, 137-148DOI: (10.1016/j.str.2007.10.027) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 3 Deuterium Accessibility for Peptides in the TCP Filament and TcpA Monomer (A) Average deuteration levels for nonoverlapping peptides in the TCP filament. Bars span the length of each peptide and are colored according to their average deuteration over six time points (10, 30, 100, 300, 1000, 3000 s), reported as a percentage of a fully deuterated peptide, as shown in the color key. The first two residues of each peptide are not shown as they are fully deuterated at all time points. The TcpA secondary structure is indicated above the graph: blue coils represent α helices and red arrows represent β strands. (B) TcpA crystal structure colored as in (A) according to its deuterium accessibility in TCP filament. Segments colored gray represent regions not covered by nonoverlapping peptides. Residues Cys120 and Cys186, which delineate the D-region, are shown as a ball-and-stick representation. The N-terminal 28 residues were modeled onto the TcpA structure using the coordinates for the PAK pilin N terminus (Craig et al., 2003). Discrete structural regions and residues used for filament modeling and/or mutational analysis are labeled. (C) Comparison of deuteration levels for identical peptides in the TcpA monomer (upper lines) and the TCP filament (lower lines), mapped onto the amino acid sequence of TcpA. The amino acid sequences are identical for the monomer and the filament for residues 29–199, with the exception of residue 181, which is an alanine in the TCP filament (TCPH181A). Secondary and other structural features are indicated above the sequence. Peptides are shown for six time points: 10, 30, 100, 300, 1000, 3000 s, from top to bottom. Each peptide is labeled by the residues it spans, with its average percent labeling over the six time points shown in parentheses. Structure 2008 16, 137-148DOI: (10.1016/j.str.2007.10.027) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 4 Refined TCP Filament Model (A) TCP model viewed as a ribbon diagram from the distal end of the filament shows the packing of the N-terminal α helices. (B) Surface representation of the TCP model shows the N-terminal α helices completely filling the filament core, leaving no solvent-filled channel for deuterium exchange. The D-regions protrude from the filament surface giving it a bumpy appearance. (C and D) (C) Side view of the TCP model as a ribbon and (D) surface representation with a single subunit outlined. Residues 13–23 of α1-N, which were shown to be readily labeled by deuterium, are colored red. His181 is shown in blue, Asp113 is yellow, Glu158 is orange, Asp129 is magenta, and additional residues in the D-region shown by Kirn et al. (2000) to be involved in pilus-pilus interactions are green (Asp175, Glu183, and Lys187). (E) Close-up of the N termini to show the putative electrostatic interaction between the positively charged N-terminal amine of Met1 and the negatively charged Glu5 side chain. (F) Close-up of the α2:α1-N/α3 interface, formed between the αβ-loop of one subunit (green) and α1-N and α3 of a neighboring subunit (yellow). The interface is shown as if looking out from the interior of the filament. (G) Close-up of the gap in the TCP filament surface, which exposes the polar face of an amphipathic segment of α1-N, residues 13–23. Key residues, including the three glycines, are labeled. Structure 2008 16, 137-148DOI: (10.1016/j.str.2007.10.027) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 5 Amino Acid Sequence Alignments of α1-N and Helical Wheel Representation of the Amphipathic Segment (A) Residues 1–30 are shown by their one-letter codes. Polar residues are shaded. Boxed residues indicate the highly deuterated segment of TcpA α1-N. Vc, V. cholerae; Ec, enterotoxigenic E. coli (CofA and LngA) and enteropathogenic E. coli (BfpA); Cr, Citrobacter rodentium; Ng, N. gonorrhoeae; Nm, N. meningitidis; Pa, P. aeruginosa; Dn, Dichelobacter nodosus; Hi, Haemophilus influenzae. (B) Helical wheel representation of residues 11–29 of TcpA. Polar residues are shaded. The polar face of the α helix corresponds to the exposed face in the TCP model, as indicated. Structure 2008 16, 137-148DOI: (10.1016/j.str.2007.10.027) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 6 Analysis of Pilin Expression, Pilus Assembly, and Pilus Functions in TcpA Mutants (A) Immunoblot showing pilin subunit expression in whole cell lysates (top panel) and pilus assembly, indicated by the presence of pilin protein in sheared cell homogenates (bottom panel). Wild-type refers to V. cholerae strain O395, classical biotype. (B) Pilin and pilus expression were also assessed by autoagglutination and phage transduction assays. (C) TEM of TCPE83R. (D) TEM of TCPR26E/E83R. Structure 2008 16, 137-148DOI: (10.1016/j.str.2007.10.027) Copyright © 2008 Elsevier Ltd Terms and Conditions