Discovery of biomarkers of endometrial receptivity through a minimally invasive approach: a validation study with implications for assisted reproduction

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Presentation transcript:

Discovery of biomarkers of endometrial receptivity through a minimally invasive approach: a validation study with implications for assisted reproduction Crystal Chan, M.D., M.Sc., Carl Virtanen, M.Sc., Neil A. Winegarden, M.Sc., Terence J. Colgan, M.D., Theodore J. Brown, Ph.D., Ellen M. Greenblatt, M.D. Fertility and Sterility Volume 100, Issue 3, Pages 810-817.e8 (September 2013) DOI: 10.1016/j.fertnstert.2013.04.047 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Unsupervised hierarchical clustering of uterine aspiration (LH + 2 and LH + 7) and endometrial biopsy (LH + 7) samples. This heat-map representation is based on the normalized intensity values of each gene, and the dendrograms demonstrate the segregation of LH + 7 and LH + 2 samples. Fertility and Sterility 2013 100, 810-817.e8DOI: (10.1016/j.fertnstert.2013.04.047) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Gastrin immunostaining in glandular epithelial cells. Gastrin staining (brown) was positive in (A) subpopulations of endometrial glandular epithelial cells of the LH + 7 biopsy samples but not of (B) surgical samples obtained from women during the proliferative phase. (C) Gastrin staining of the stomach antrum, a well-established gastrin-producing tissue. Inset figures in A and C represent secondary antibody-only controls. Bars represent 20 μm with the exception of inset A, where it represents 100 μm. Fertility and Sterility 2013 100, 810-817.e8DOI: (10.1016/j.fertnstert.2013.04.047) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Principal component analyses on samples from (A) the aspiration data set and (B) the GSE4888 data set using probes associated with the predictor cassette to illustrate the degree of separation between sample types. For the aspiration samples, principal component X represents 88.8% of the variation, Y 2.41%, and Z 1.49%. For the GSE4888 samples, principal component X represents 53.52% of the variation, Y 14.8%, and Z 11.19%. Red: LH + 7 and MSE samples. Blue: LH + 2 and ESE samples. Fertility and Sterility 2013 100, 810-817.e8DOI: (10.1016/j.fertnstert.2013.04.047) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions