HIV-1-infected patients with focal neurologic signs: diagnostic role of PCR for Toxoplasma gondii, Epstein-Barr virus, and JC virus Hans H. Hirsch, Pascal.

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Presentation transcript:

HIV-1-infected patients with focal neurologic signs: diagnostic role of PCR for Toxoplasma gondii, Epstein-Barr virus, and JC virus Hans H. Hirsch, Pascal R.A. Meylan, Werner Zimmerli, Anne Iten, Manuel Battegay, Peter Erb Clinical Microbiology and Infection Volume 4, Issue 10, Pages 577-584 (October 1998) DOI: 10.1111/j.1469-0691.1998.tb00040.x Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 PCR analysis of cerebrospinal fluid. Representative CSF results arc shown for patients with PML (Patient 1), TE (Patient 2) and PCL (Patient 3). The PCR was analyzed on 2.2% agarose electrophoresis gels stained with ethidium bromide. The respective amplicons of JC virus (JCV), Toxoplasma gondii (TOX) and Epstein–Barr virus (EBV) arc indicated (arrows). The external controls consisted of peripheral blood leukocyte (PBL) DNA, high and low positive controls as defined for each target in the methods section, and water. The standard diagnostic consists of duplicates of the patient specimen, flanked by water as negative controls, and the patient specimen spiked with the target as internal amplification control. Clinical Microbiology and Infection 1998 4, 577-584DOI: (10.1111/j.1469-0691.1998.tb00040.x) Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions