Volume 55, Issue 2, Pages 667-673 (February 1999) Hyaluronan decreases peritoneal fluid absorption: Effect of molecular weight and concentration of hyaluronan  Tao Wang, Hui-Hong Cheng, Olof Heimbürger, Chi Chen, Jacek Waniewski, Jonas Bergström, Bengt Lindholm  Kidney International  Volume 55, Issue 2, Pages 667-673 (February 1999) DOI: 10.1046/j.1523-1755.1999.00279.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Intraperitoneal volume versus time: Effect of different of molecular weights and concentrations of hyaluronan. Symbols are: (□) control group (N = 6); (◊) HA85K group (N = 6); (○) HA280K group (N = 6); (▪) HA500K group (0.01% HA group; N = 6); (○) HA4M group (N = 6); () 0.05% HA group (N = 6); (○) 0.1% HA group (N = 6); () 0.5% HA group (N = 6). Kidney International 1999 55, 667-673DOI: (10.1046/j.1523-1755.1999.00279.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 2 The RISA elimination rate from the peritoneal cavity. Abbreviations are: KE, the total RISA elimination rate representing the fluid absorption rate from the peritoneal cavity; KEB, the RISA elimination rate to the blood from the peritoneal cavity representing the peritoneal lymphatic absorption; KET, the RISA elimination rate to the peritoneal tissue. Symbols are: (□) control group; (▨) HA85K group; (▧) HA280K group; (▪) HA500K group (0.01% HA group); (▤) HA4M group; () 0.05% HA group; () 0.1% HA group; () 0.5% HA group. Significant differences are marked. *P < 0.05 compared with the control group; **P < 0.01 compared with the control group (mean±sd). Kidney International 1999 55, 667-673DOI: (10.1046/j.1523-1755.1999.00279.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 3 Transcapillary ultrafiltration rate between 3minutes and 240minutes of the dwell. (a) P < 0.05 compared with the control and HA500K groups. (b) P < 0.05 compared with the control, 0.01% HA, and 0.5% HA groups. (c) P < 0.05 compared with the 0.5% HA group. Symbols are the same as in Figure 2. Kidney International 1999 55, 667-673DOI: (10.1046/j.1523-1755.1999.00279.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 4 Schematic description of the effect of different molecular weights and different concentrations of hyaluronan (in the peritoneal membrane or within the filter “cake”) on the peritoneal fluid absorption. Abbreviations are: Ci, the concentration of hyaluronan; Mi, the molecular weight of hyaluronan. (Modified from Berriaud et al11.) Kidney International 1999 55, 667-673DOI: (10.1046/j.1523-1755.1999.00279.x) Copyright © 1999 International Society of Nephrology Terms and Conditions