Volume 84, Issue 3, Pages (February 1996)

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Volume 84, Issue 3, Pages 451-459 (February 1996) The Tomato Cf-2 Disease Resistance Locus Comprises Two Functional Genes Encoding Leucine-Rich Repeat Proteins  Mark S Dixon, David A Jones, James S Keddie, Colwyn M Thomas, Kate Harrison, Jonathan D.G Jones  Cell  Volume 84, Issue 3, Pages 451-459 (February 1996) DOI: 10.1016/S0092-8674(00)81290-8

Figure 1 Analysis of a Recombinant between Cf-2 and Cf-5 (A) Schematic representations of regions of chromosome 6 are shown for key plants in the crosses used to identify the rare recombinant between Cf-2 and Cf-5 (V454). The linear order of closely linked RFLP probes are shown, and the genotype of each marker is denoted by superscripted text. The 0/5 superscript indicates that no polymorphism has been identified that reveals a difference between the Cf0 and Cf5 NILs; the question mark indicates that the marker reveals a pattern that does not conform entirely to that of any of the parental genotypes. F1 designates the plant derived from crossing Cf2 to Cf5, while V454 indicates the recombinant chromosome identified in the single disease-sensitive plant (V454) among 12,000 progeny of a test cross of the F1 heterozygote to Cf0. (B) Hybridization of the RFLP marker SC3-8 to Cf0 (0), Cf2 (2), Cf5 (5), and a homozygote for the V454 recombinant chromosome (V). Closed arrowheads indicate the positions of bands specific for L. pimpinellifolium DNA introgressed with Cf-2. Molecular weights in kilobases are indicated to the left. (C) The same blot as in (B) hybridized with a 5.5 kb HindIII probe derived from Cf0 encompassing part of the region designated as MG112B together with some flanking sequences. Closed arrowheads identify Cf2-specific bands that are present in the recombinant V454, and the open arrowhead identifies a novel band not present in either the Cf2 or Cf5 parents. Cell 1996 84, 451-459DOI: (10.1016/S0092-8674(00)81290-8)

Figure 2 Complementation with Cosmids from the Cf-2 Locus (A) Map of SacI (S) and XhoI (X) restriction endonuclease sites in the region carrying overlapping cosmids that hybridize to MG112. Closed boxes indicate the regions (A and B) that hybridize with the RFLP marker MG112, while stippled boxes indicate the regions that hybridize with the 5.5 kb HindIII probe derived from Cf0 (probe C) used for Southern blot analysis in Figure 1C. (B) Cosmid contig spanning the Cf-2 locus. Horizontal lines represent the inserts contained within eight independent MG112-hybridizing cosmid clones. The circled letters R and S identify cosmids or subclones that either confer resistance or fail to confer resistance (susceptible), respectively, upon transformation. The scale bar indicates 5 kb. (C) Sequenced regions of cosmid 94 are shown as rectangles. Hatching indicates the transcribed regions, and closed boxes indicate introns. Horizontal arrows show the direction of transcription. Broken lines indicate the location of the sequenced regions with respect to cosmid 94. (D) Leaves of wild-type plants and plants transformed with cosmid 94 after challenge with C. fulvum race 4. Shown are Cf0 (a), Cf2 (b), and the first five independent transformants with cosmid 94 (c–g). Southern blot analysis indicates that transformants shown in (c) and (d) do not contain an intact copy of Cf-2. Cell 1996 84, 451-459DOI: (10.1016/S0092-8674(00)81290-8)

Figure 3 Primary Structure of the Cf-2 Proteins The amino acid sequences predicted from the DNA sequence of Cf-2.2 are shown divided into seven domains (A–G), as described in the text. In domain C, the conserved L of the LRRs is often replaced by V, F, I, or M. These are highlighted in blue, while other conserved amino acids are shown in red. In domains E and G, acidic amino acids are highlighted in green, and the basic amino acids are in purple. Potential N-linked glycosylation sites are underlined. The three amino acid differences in Cf-2.1 are placed above their corresponding position in the sequence of Cf-2.2. Numbers to the right of domain C indicate the specific LRR number. Letters A and B to the right of the sequence indicate the type of LRR (see text). Cell 1996 84, 451-459DOI: (10.1016/S0092-8674(00)81290-8)

Figure 4 Analysis of Repeated and Homologous Sequences within Cf-2 and Cf-9 (A–C) The Cf-2.1 and Cf-9 protein sequences were compared with themselves and each other using the Genetics Computer Group program COMPARE (University of Wisconsin). A window of 24 and a stringency of 18 (75% similarity) was used for each analysis, and results are displayed as diagon plots. The limits of domain C (the LRR region) in each protein sequence are indicated on each axis. The N-terminus of each protein is aligned with the origin of each graph. (A) The Cf-2.1 protein compared with itself. (B) The Cf-9 protein compared with itself. (C) Comparison of Cf-2.1 protein with Cf-9 protein. Arrows identify the extent of the high homology in the C-terminal region (see text). (D) The Cf-2.1 nucleic acid sequence encompassing the entire open reading frame was compared with itself. A window of 72 (one complete LRR) and a stringency of 54 (75% identity) was chosen. The positions for the starts of particular LRRs are indicated on each axis. Cell 1996 84, 451-459DOI: (10.1016/S0092-8674(00)81290-8)

Figure 5 The C-Terminal Portions of the Cf-2.1 and Cf-9 Proteins Exhibit Strong Similarity The C-terminal portions, including the last 12 LRRs, of the predicted Cf-2.1 and Cf-9 proteins are aligned by the GAP program from the Genetics Computer Group. Vertical lines represent identity between a corresponding pair of amino acid residues, a colon represents similarity, a single dot represents a low level of similarity, and a gap represents no similarity. C to G represent the domains shown in Figure 3 and described in the text. Amino acids shown in bold identify regions of identity discussed in the text. Cell 1996 84, 451-459DOI: (10.1016/S0092-8674(00)81290-8)

Figure 6 Two Models for Mechanisms by Which Cf-2 and Cf-9 Might Activate the Defense Response upon Avr Product Recognition PK, transmembrane protein kinase; PM, plasma membrane. In model (A), upon Avr recognition by a Cf gene product, the resulting complex might directly interact with a membrane-bound NADPH oxidase, which produces superoxide anions and initiates the plant defense response. In model (B), upon Avr product binding, the resulting complex interacts with a transmembrane protein kinase analogous to TMK1 or RLK5 (Chang et al. 1992; Walker 1994) or to Xa21 (Song et al. 1995). Kinase action then triggers subsequent events that activate an NADPH oxidase. Cell 1996 84, 451-459DOI: (10.1016/S0092-8674(00)81290-8)