EBV Selection Required less time to apply genotype body composition Association of melanocortin 4 receptor (MC4R) polymorphism with body composition and carcass traits of commercial pigs in Thailand body composition carcass traits EBV genotype Selection Required less time to apply

SL, shoulder length (cm) Association of melanocortin 4 receptor (MC4R) polymorphism with body composition and carcass traits of commercial pigs in Thailand Traits for study BL, body length (cm) SL, shoulder length (cm) HL, ham length (cm) RL, rump length %Lean, percent lean aLAM, loin eye area muscle adjust at 104 kg (kg) D104, day during birth to body weight at 104 kg (day) aBF, backfat thickness adjust at 104 kg (cm) back

digested by the restriction endonuclease Taq I Association of melanocortin 4 receptor (MC4R) polymorphism with body composition and carcass traits of commercial pigs in Thailand digested by the restriction endonuclease Taq I AG GG AA 483 bp 500 bp 407 bp 76 bp Figure 2 RFLP fingerprint on 2% agarose. AA = 483 bp AG = 483,407 and 76, GG = 407,76

rg Low to Moderate Genetic response slow Table1. Heritability of the traits (diagonal), genetic correlation (above diagonal) and phenotypic correlation (belowdiagonal) rg Low to Moderate Traits BL SL RL HL aBF aLAM %Lean D104 0.07 -0.08 -0.11 -0.21 0.30 -0.35 -0.44 -0.47 0.14 0.53 0.77 -0.22 0.33 0.35 -0.38 0.06 0.38 0.55 0.15 0.04 -0.07 -0.31 0.11 0.56 0.39 0.19 -0.37 0.26 0.40 -0.18 0.01 0.02 0.08 -0.06 0.29 -0.17 -0.73 -0.16 0.09 0.21 0.79 -0.03 -0.14 0.03 -0.54 0.22 -0.28 -0.25 -0.05 0.13 Genetic response slow 1 BL, body length (cm); SL, shoulder length (cm); RL, rump length; HL, ham length (cm); aBF, backfat thickness adjust at 104 kg (cm); aLAM, loin eye area muscle adjust at 104 kg (kg); %Lean, percent lean; D104, day during birth to body weight at 104 kg (day).

Conclusions h2 Low 1) Body composition and D104 2) Body composition rg Association of melanocortin 4 receptor (MC4R) polymorphism with body composition and carcass traits of commercial pigs in Thailand Conclusions h2 Low Slow 1) Body composition and D104 year 2) Body composition rg Low to Moderate 3) Polymorphism of MC4R may not be appropriate to use as candidate gene in this populations - to confirm the result, many sample must be included

? ? ? Introduction Candidate genes approach Good meat texture Low cholesterol Good taste Price higher 2-3 times Thai Native Chickens Low growth rate Broiler ? ? ? Improve growth traits Growth traits Candidate genes approach (Wattanachant et al., 2004; Jaturasitha et al., 2008)

Average Daily Gain (ADG) Materials and methods Pradoo Hang Dam Chee (60 chickens/breed) Data Body Weight (0, 4, 8, 12, 16 Wks) Average Daily Gain (ADG) (0- 4, 0-8, 0-12, 0-16 Wks)

Results and Discussion Marker- traits Analysis IGF-I cGH IGFBP2 Not Associated GHSR T>C A>C G>A C>T Intron1 5’ UTR Intron3 Intron2 Not Associated EBV data Pradoo Hang Dam Chee

Conclusions Chee Pradoo Hang Dam Improve growth traits IGF-I cGH Not Associated Chee Pradoo Hang Dam IGF-I cGH marker-assisted selection Improve growth traits