A multicenter evaluation of genotypic methods for the epidemiologic typing of Legionella pneumophila serogroup 1: results of a pan-European study Norman.

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A multicenter evaluation of genotypic methods for the epidemiologic typing of Legionella pneumophila serogroup 1: results of a pan-European study Norman K. Fry, Stella Alexiou-Daniel, Jette M. Bangsborg, Sverker Bernander, Maddalena Castellani Pastoris, Jerome Etienne, Benita Forsblom, Valeria Gaia, Jörgen H. Helbig, Diane Lindsay, P. Christian Löck, Carmen Pelaz, SØren A. Uldum, Timothy G. Harrison Clinical Microbiology and Infection Volume 5, Issue 8, Pages 462-477 (August 1999) DOI: 10.1111/j.1469-0691.1999.tb00176.x Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Strategy adopted by the European Working Group on Legionella Infections (EWGLI) for the epidemiologic typing of strains examined in the investigation of travel-associated legionellosis. NA=not applicable Clinical Microbiology and Infection 1999 5, 462-477DOI: (10.1111/j.1469-0691.1999.tb00176.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Examples of the patterns obtained with a selection of the genotypic methods with the four sets of ‘epidemiologically related’ strains of L. pneumophila sg 1 (subdivision I). The four epidemiologically related sets comprise study code numbers 2 and 62, 37, 42 and 47, 43 and 110, and 44, 77 and 82. Where results from different gels or blots are combined, markers from each gel/blot are included. See text for details of methods. (A) Electrophoretic results of restriction endonuclease analysis (REA) with HaeIII. (B) Electrophoretic results following PFGE III with SfiI. (C) Electrophoretic results following PFGE VI with NotI. (D) Southern blots following NciI digests with RFLP I. (E) Southern blots following HindIII digestion with ribotyping I. (F) PCR DNA fingerprinting using an arbitrary (BG2) and repeat sequence primer (ERIC2). (G) Electrophoretic results of AFLP analysis using PstI with selective primer AFLP-PstI-G. Clinical Microbiology and Infection 1999 5, 462-477DOI: (10.1111/j.1469-0691.1999.tb00176.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions