Endothelial Adherens Junctions Peter Petzelbauer, Thomas Halama, Marion Gröger  Journal of Investigative Dermatology Symposium Proceedings  Volume 5, Issue 1, Pages 10-13 (December 2000) DOI: 10.1046/j.1087-0024.2000.00002.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Three color immunofluorescent staining of endothelial cells grown as monolayers on tissue culture plastic. Laser scan image, scale bar: 10 μm. Actin fibers are stretched from one cell border to the other and form a delicate network of parallel-bundled fibers. VE-cadherin (cad-5) and β-catenin (B-CAT) colocalize at the cell border forming an interdigitated and belt-like junction zone between cells. Journal of Investigative Dermatology Symposium Proceedings 2000 5, 10-13DOI: (10.1046/j.1087-0024.2000.00002.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Transmission microscopy of ECV304 cells, stable transfected with VE-cadherin, PECAM-1. or vector alone.Halama et al. (1999). Cells were cultured in RPMI/10% fetal calf serum (A, B, C) or in RPMI/10% fetal calf serum/20 ng basic FGF. Whereas in the absence of exogenous growth factors, both VE-cadherin- and PECAM-1-transfectants cluster, FGF induces cell scattering in VE-cadherin, but not in the PECAM-1 transfectants. Vector only cells scatter, whether FGF is present or not. Journal of Investigative Dermatology Symposium Proceedings 2000 5, 10-13DOI: (10.1046/j.1087-0024.2000.00002.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions