Analysis of GPCR deletion strains.

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Gene Primer Forward Primer Reverse Eficiency (%) Slope R2

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Analysis of GPCR deletion strains. Analysis of GPCR deletion strains. (A) Growth of GPCR deletion mutants on rich medium versus minimal medium with cellulose as the carbon source. Strains were grown on 3% (wt/vol) malt extract agar plates (MEX) or Mandels-Andreotti agar plates with 1% (wt/vol) carboxymethylcellulose (CMC) for 7 days at room temperature in daylight (LD) or constant darkness (DD). Inoculation was done at the edge of the plates to allow several days of measurement. A picture representative of results from 3 biological replicates is shown. (B) cbh1 transcript levels of the Δcsg1, Δcsg2, Δ37525, and Δ55561 mutants were determined by quantitative RT-PCR after growth on cellulose in constant darkness after 48, 72, and 96 h and are shown relative to wild-type (WT) QM6a results (*, P ≤ 0.01). (C) Specific cellulase activity in culture filtrates after growth on cellulose for 72 or 96 h in constant darkness. Activity data are given relative to that of the wild-type QM6a strain (*, P ≤ 0.01; **, P ≤ 0.001). (D) cbh1 transcript levels of the Δcsg1 and Δcsg2 mutants after growth on lactose in constant darkness after 40 h are shown relative to the wild-type results. (E) Specific cellulase activity in culture filtrates after growth on lactose for 40 h in constant darkness. Activity is given relative to the wild-type results. Eva Stappler et al. mSphere 2017; doi:10.1128/mSphere.00089-17