Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA. A, relative quantitation over three.

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Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA. A, relative quantitation over three injection replicates for diphosphopeptide, YHGHpS293MSDPGVpS300YR, with precursor ion M extracted at m/z 876.8155. Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA.A, relative quantitation over three injection replicates for diphosphopeptide, YHGHpS293MSDPGVpS300YR, with precursor ion M extracted at m/z 876.8155. B, relative quantitation for the corresponding phosphopeptides, YHGHpS293MSDPGVSYR and/or YHGHSMSDPGVpS300YR, with precursor ion M extracted at m/z 836.8323. C, phosphopeptide YGMGTpS232VER with precursor ion M extracted at m/z 540.2150. D, independent Western blot analysis of PDHE1α phosphorylation with phosphosite-specific and total protein antibodies in response to DCA treatment. Birgit Schilling et al. Mol Cell Proteomics 2012;11:202-214 © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.