Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to rpoB gene sequencing for species identification.

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Presentation transcript:

Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to rpoB gene sequencing for species identification of bloodstream infection staphylococcal isolates T. Spanu, E. De Carolis, B. Fiori, M. Sanguinetti, T. D'Inzeo, G. Fadda, B. Posteraro Clinical Microbiology and Infection Volume 17, Issue 1, Pages 44-49 (January 2011) DOI: 10.1111/j.1469-0691.2010.03181.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Score oriented dendrogram showing the spectral similarities of staphylococcal type strains (n = 46), as obtained by Microflex LT (Bruker Daltonik GmbH). Clinical Microbiology and Infection 2011 17, 44-49DOI: (10.1111/j.1469-0691.2010.03181.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry of (a) Staphylococcus hominis subsp. novobiosepticum, (b) Staphylococcus hominis subsp. hominis, (c) Staphylococcus capitis subsp. capitis and (d) Staphylococcus capitis subsp. urealyticus, showing the differences among the subspecies. The absolute intensities of the ions are shown on the y-axis, and the masses (Da) of the ions are shown on the x-axis. The m/z value represents the mass to charge ratio. Clinical Microbiology and Infection 2011 17, 44-49DOI: (10.1111/j.1469-0691.2010.03181.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions