Dependence of DesII steady-state parameters V (●) and (○) on pH.

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Presentation transcript:

Dependence of DesII steady-state parameters V (●) and (○) on pH. Dependence of DesII steady-state parameters V (●) and (○) on pH. The ordinate denotes the base-10 logarithm of the steady-state parameter (P = V or ) normalized vs. the minimum value observed. The results for V are shifted one log unit along the ordinate for presentation purposes. Error bars denote ±1 SE of the observed parameter at the given pH following propagation through the logarithm. Fitted lines and the apparent pKa values were estimated by weighted nonlinear regression as described in SI Kinetic Analysis. Mark W. Ruszczycky et al. PNAS 2013;110:6:2088-2093 ©2013 by National Academy of Sciences