Examples of cancer tissue profiles.

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Examples of cancer tissue profiles. Examples of cancer tissue profiles. The figure shows staining patterns for four proteins with two images for each protein. The antibody HPR000704 generated from the MTHFD1 gene located on chromosome 14 shows positivity in squamous epithelium restricted to the less differentiated basal cells as exemplified in oral mucosa (a). A similar pattern of positive staining was most abundant in the less well differentiated cancer cells in squamous cell carcinoma from the head and neck region (b). The antibody HPR000637 generated from the PABP2gene located on chromosome 14 shows distinct positive nuclei in epithelial cells as well as in inflammatory and stromal cells in normal mucosa from the rectum (c). In colorectal cancer strong nuclear staining is apparent in large, atypical cancer cells as well as in mesenchymal cells of cancer stroma (d). The antibody HPR000837 generated toward the TCN2 gene located on chromosome 22 shows positive staining of the basal layer in normal urothelium from the urinary bladder (e). Several cancer tissues show a weak to moderate cytoplasmic staining with highest levels of expression found in urothelial carcinoma (f). The antibody HPR000527 generated toward a completely unknown protein, NP_071381.1, whose gene is located on chromosome 22, shows very weak staining in the epithelial cells of breast lobuli, whereas terminal duct cells show a slightly stronger staining (g). Breast carcinomas were in general positive with a stronger intensity of immunoreactivity (h). Mathias Uhlén et al. Mol Cell Proteomics 2005;4:1920-1932 The American Society for Biochemistry and Molecular Biology