Matrix-Induced Autologous Chondrocyte Implantation With Autologous Bone Grafting for Osteochondral Lesions of the Femoral Trochlea  Kristofer J. Jones, M.D., Brian M. Cash, M.D.  Arthroscopy Techniques  Volume 8, Issue 3, Pages e259-e266 (March 2019) DOI: 10.1016/j.eats.2018.10.022 Copyright © 2018 Arthroscopy Association of North America Terms and Conditions

Fig 1 (A) Preoperative magnetic resonance imaging of the left knee reveals a 1.5-cm region of full-thickness cartilage loss in the femoral trochlea (arrow) on the sagittal view. Underlying the lesion is a 6 × 8-mm subchondral cyst (arrow) that can be seen on sagittal (B), coronal (C), and axial (D) views. Arthroscopy Techniques 2019 8, e259-e266DOI: (10.1016/j.eats.2018.10.022) Copyright © 2018 Arthroscopy Association of North America Terms and Conditions

Fig 2 (A) During the first of this 2-stage procedure, a large ring curette is used to harvest 200 to 300 mg of hyaline cartilage (arrow) from the intercondylar notch along the lateral femoral condyle of the left knee with the knee flexed. An anterolateral portal is used for viewing, and the curette is inserted through the anteromedial portal. (B) A diagnostic arthroscopy is also performed to assess the trochlear lesion (arrows) with regard to size, depth, and morphology. This view is from an anterolateral portal with the knee extended. Arthroscopy Techniques 2019 8, e259-e266DOI: (10.1016/j.eats.2018.10.022) Copyright © 2018 Arthroscopy Association of North America Terms and Conditions

Fig 3 (A) With the patient supine and left knee slightly flexed, the full-thickness lesion in the femoral trochlea is exposed (arrow) through a medial parapatellar arthrotomy. (B) The borders of the lesion (arrow) are sharply delineated using a 15-blade scalpel. (C) A ring curette is used to debride the base of the defect and create stable vertical edges at the periphery of the lesion (arrow). It is important to remove the underlying calcified cartilage layer without penetrating the subchondral bone. (D) A 2.7-mm drill is used to perforate the lesion and gain access to the underlying bone defect (arrow). The sclerotic margins of the defect are debrided with a curette and drilled with a k-wire. Arthroscopy Techniques 2019 8, e259-e266DOI: (10.1016/j.eats.2018.10.022) Copyright © 2018 Arthroscopy Association of North America Terms and Conditions

Fig 4 (A) With the patient supine and left knee slightly flexed, a template is made by placing a sterile foil wrapper from any commercial suture package into the defect and pressing along the edges of the defect (arrows) with a Freer elevator. (B) Once cut, the template can be checked for accurate sizing in the defect, with the 12 o'clock position marked for orientation. After the tourniquet is released, hemostasis at the defect site is achieved with epinephrine-soaked neural patties (C) or thrombin-soaked gel foam (D) (arrows). Arthroscopy Techniques 2019 8, e259-e266DOI: (10.1016/j.eats.2018.10.022) Copyright © 2018 Arthroscopy Association of North America Terms and Conditions

Fig 5 (A) The MACI scaffold (arrows) is placed on a Tegaderm dressing to provide a stable platform for graft preparation. When the notch is in the lower left corner of the scaffold, the cellular side is facing up. It is imperative to note proper orientation before cutting the graft. (B) The template is placed between the Tegaderm and the scaffold, and the scaffold is cut using tenotomy scissors. It is helpful to have an assistant hold the corners of the graft and Tegaderm stable with small forceps while cutting. (MACI, matrix-induced autologous chondrocyte implantation.) Arthroscopy Techniques 2019 8, e259-e266DOI: (10.1016/j.eats.2018.10.022) Copyright © 2018 Arthroscopy Association of North America Terms and Conditions

Fig 6 (A) With the patient supine and left knee slightly flexed, the defect is filled with BMAC-soaked bone graft to a level flush with the subchondral bone (arrow). (B) The base of the defect is filled with Tisseel fibrin glue. (C) The scaffold is placed flush into the lesion with the cell side down. It is helpful to insert the scaffold with it still attached to the foil template (arrow), to allow for stable insertion in the proper orientation. (D) After removing the template and holding light digital pressure on the graft for 3 minutes, the fibrin glue has solidified and the graft (arrow) is now secured. (BMAC, bone marrow aspirate concentrate.) Arthroscopy Techniques 2019 8, e259-e266DOI: (10.1016/j.eats.2018.10.022) Copyright © 2018 Arthroscopy Association of North America Terms and Conditions