A Possible Vertical Transmission of Human Papillomavirus Genotypes Associated with Epidermodysplasia Verruciformis Michel Favre, Slavomir Majewski, Nando.

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A Possible Vertical Transmission of Human Papillomavirus Genotypes Associated with Epidermodysplasia Verruciformis Michel Favre, Slavomir Majewski, Nando De Jesus, Magdalena Malejczyk, Gérard Orth, Stefania Jablonska Journal of Investigative Dermatology Volume 111, Issue 2, Pages 333-336 (August 1998) DOI: 10.1046/j.1523-1747.1998.00312.x Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Detection of EV HPV DNA sequences. DNA from various specimens taken from the mother was analyzed by nested PCR using the CP65/CP70 and CP66/CP69 primers aimed at the detection of EV HPV (Berkhout et al. 1995) and primers specific for HPV types 5, 8, 24, 36, and 3. Positive controls were cloned HPV DNA as indicated and cloned HPV5 DNA for EV HPV detected with CP65/CP70 and CP66/CP69 primers. DNA was omitted in the PCR reaction mixture as a negative control. Amplification products obtained after the first round (primers CP65/CP70) and the second round (primers CP66/CP69 or specific primers 3/4) of PCR were analyzed by agarose gel electrophoresis and detected after ethidium bromide staining of the gels. The adequacy of cellular DNA preparations was demonstrated by PCR amplification of β-globin sequences (Greer et al. 1991). Journal of Investigative Dermatology 1998 111, 333-336DOI: (10.1046/j.1523-1747.1998.00312.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions