Fig. 2 Identification of the candidate AvrSr35 gene.

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Fig. 2 Identification of the candidate AvrSr35 gene. Identification of the candidate AvrSr35 gene. (A) EMS-induced mutations and MITE insertion site in the AvrSr35 gene. (B) Phylogeny of the AvrSr35 gene in diverse Pgt isolates. The colored tree tips correspond to alleles originating from the Sr35-avirulent (red) and Sr35-virulent (blue) isolates. The sequences from the Pgt isolates heterozygous at the AvrSr35 locus are marked with stars. The different AvrSr35 alleles from these isolates have A or V appended to the sequence name. The sequences with MITE insertion are marked by checkmarks. The AvrSr35 gene sequences form two major clades, A and V. Clade V includes the virulent allele with the MITE insertion. Bootstrap values above 0.7 are shown on the tree nodes. The second AvrSr35 allele (accession number MF596174) from isolate 99KS76A-1 with a nonsense mutation in coding sequence was used as an outgroup. Andres Salcedo et al. Science 2017;358:1604-1606 Published by AAAS