How was cps-6 identified as a factor important for paternal mitochondrial elimination ? By genetic screen By candidate-based RNAi screen By educational guess By pure luck By biochemical purification

Where does cps-6 act to promote PME? In the oocyte In paternal mitochondria In maternal mitochondria In the autophagosome In the nucleus of the oocyte

Based on the results in the paper, CPS-6 is important for Degradation of paternal mtDNA Recruitment of the autophagosome to paternal mitochondria Degradation of paternal mitochondrial proteins All of the above A & B

How are paternal mitochondria selectively targeted for degradation in the fertilized eggs? A. They look different from maternal mitochondria B. Both paternal and maternal mitochondria are targeted for degradation C. Paternal mitochondria are depolarized and damaged soon after their entry into the oocyte and thus marked for degradation Paternal mitochondria are smaller than maternal mitochondria and thus are easier to be degraded by the autophagosome Paternal mitochondria are larger than maternal mitochondria and thus can be readily recognized and degraded.

What are the important questions that remain to be addressed?

Why paternal mitochondria need to be eliminated from fertilized eggs ? A. They are larger than maternal mitochondria and thus not compatible Maternal mitochondria can do without paternal mitochondria They have a higher frequency of mutations and thus are evolutionarily undesirable for embryo development They actually don’t need to be eliminated. None of the above