Predicted microbiota age against the actual age of visitors and villagers. Predicted microbiota age against the actual age of visitors and villagers. (A) Fecal samples. (B) Nasal samples. (C) Oral samples. (D) Skin samples. The prediction was made by constructing a random forest model using the villagers as the training set. The blue line in each panel illustrates a simple linear regression curve using samples from the training set. Data points for visitors are from multiple dates. Kelly V. Ruggles et al. mSphere 2018; doi:10.1128/mSphere.00193-18