High-affinity iron permease (FTR1) gene sequence-based molecular identification of clinically important Zygomycetes I. Nyilasi, T. Papp, Á. Csernetics,

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High-affinity iron permease (FTR1) gene sequence-based molecular identification of clinically important Zygomycetes I. Nyilasi, T. Papp, Á. Csernetics, K. Krizsán, E. Nagy, C. Vágvölgyi Clinical Microbiology and Infection Volume 14, Issue 4, Pages 393-397 (April 2008) DOI: 10.1111/j.1469-0691.2007.01932.x Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Examples of PCR amplifications demonstrating the specificity of the primers for Rhizopus schipperae, Rhizopus microsporus var. rhizopodiformis, Rhizopus oryzae and R. microsporus var. oligosporus (A—E) and PCR—restriction fragment length polymorphism (RFLP) patterns of the corresponding strains following AluI digestion of the FTR1 fragments (F—G). (A—D) Amplifications with the primer pairs Rsc1—Rsc2 (A), Rhr1—Rhr2 (B), Rho1—Rho2 (C) and Rhm1—Rhm2 (D). The order of the samples is the same in (A—D): pUC mix; CBS 138.95; UHF 3053; CBS 220.92; CBS 102.277; NRRL 514; CBS 403.51; and CBS 146.90. (E) Results of amplification with primer pairs Rsc1—Rsc2 (lanes 2–5), Rhr1—Rhr2 (lanes 6–9), Rho1—Rho2 (lanes 10–13) and Rhm1—Rhm2 (lanes 14–17); lanes: 1, pUC mix; 2, CBS 138.95; 3, 7, 11 and 15, Candida albicans ATCC 10231; 4, 8, 12 and 16, Saccharomyces cerevisiae CBS 1171; 5, 9, 13 and 17, Aspergillus fumigatus SZMC 1389; 6, CBS 220.92; 10, CBS 146.90; and 14, NRRL 514. (F, G) PCR-RFLP patterns of the strains involved in the study. (F) Lanes 1–11: pUC mix; CBS 220.92; CBS 102.277; CBS 138.95; UHF 3053; NRRL 1422; ATCC 24905; FRR 2109; ATCC 42423; CBS 277.49; NRRL 3640. (G) Lanes 1–17: NRRL 514; CBS 395.54; SZMC 8100; CBS 146.90; SZMC 0497; NRRL 2908; CBS 112.07; CBS 260.28; TJM 24B2; CBS 109.939; CBS 403.51; CBS 347.49; CBS 320.35; CBS 360.92; WRLCN(M) 231; SZMC 2011; pUC mix. The fragment sizes of the pUC mix marker were 1118, 881, 692, 501/489, 404, 331, 242, 190, 147, 111/110 and 67 bp, respectively. Source of strains: ATCC, American Type Culture Collection; CBS, Centraalbureau voor Schimmelcultures, The Netherlands; NRRL, Agricultural Research Service Culture Collection, USA; SZMC, Szeged Microbial Collection, Hungary; TJM, T. J. Michailides, University of California, USA; WRLCN, Wellcome Bacterial Collection, UK; FRR, CSIRO Food Research Culture Collection, Australia; UH, Fungal Testing Laboratory, University of Texas Health Science Center, USA. Clinical Microbiology and Infection 2008 14, 393-397DOI: (10.1111/j.1469-0691.2007.01932.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions