Two-dimensional gel silver staining and two-dimensional immunoblotting using antibody to 3-nitrotyrosine. Two-dimensional gel silver staining and two-dimensional.

Slides:

Advertisements

Similar presentations

2D PAGE/Western blotting analysis for the identification of possible post-translation modification of Hsp27 from the spinal cord tissue of the SCI rat.

Advertisements

binding sites 58 of the 473 unambiguously assigned phosphorylation sites are predicted by Scansite to be sites for binding. 50 of these correspond.

Sequence alignment of C-terminal phosphorylated plant aquaporins

Fast protein LC IMAC purification of cytosolic phosphoproteins

Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.

A, high resolution MS/MS spectrum (lower panel) of 1435

Illustration of matched (FL-IFN-γR2/GFP and IFN-γR1/EBFP) and mismatched (IFN-γR1/EBFP and FL-IL-10R2/GFP) pairs of receptors. Illustration of matched.

Comparison of protein synthesis patterns of L

Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,

Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA. A, relative quantitation over three.

Two-dimensional electrophoresis results and validation with Western blotting. Two-dimensional electrophoresis results and validation with Western blotting.

Distributions of the ELDP values and Mascot scores for all protein identifications.a, frequency of ELDP value returned by correct (gray bars) and incorrect.

Significantly enriched phosphorylation motifs from up-regulated phosphopeptides by Motif-X analysis. Significantly enriched phosphorylation motifs from.

Frequency distribution of the effect size measure (ESM) of sperm protein spots of type-1 diabetic (A), type-2 diabetic (B) and non-diabetic obese (C) patients.

Schematic illustration of the assembly and interactions of sperm-bound eppin protein complex (EPC) components. Schematic illustration of the assembly and.

Novel p53 target genes identified by RNA-Seq, pSILAC and ChIP-Seq.

Assay of NOS activity. Assay of NOS activity. Box show total NOS activity, the calcium-dependent activity of the constitutive isoforms of NOS (eNOS and.

Relative abundance of proteins identified in MALDI IMS

Success rates in validation of antibodies from external providers

Mass spectrometry identification of spots in 2D blue native gels of cytoplasmic membranes isolated from BL21(DE3)pLysS Spots in 2D BN gels of cytoplasmic.

Results from the Morris water task.

A, myelinated nerve fibers in the control and biopsied mouse striatum were stained with anti-MBP antibody. A, myelinated nerve fibers in the control and.

NanoLC-MS/MS/based analysis of proteome differences between colonospheres and isogenic differentiated tumor cells. NanoLC-MS/MS/based analysis of proteome.

The evolutionary conservation of the phosphoproteomes.a, E. coli. b, B. subtilis. The evolutionary conservation of the phosphoproteomes.a, E. coli. b,

Colonopshere-enriched proteins display functional interactions.

A, schematic presentation of fetuin-A domains.

Proteins previously reported in published MALDI IMS studies and their frequency of observation in the present study. Proteins previously reported in published.

Characterization of aggregates isolated from E

Representative 2-D gel images of UC plasma from AGA (A) and IUGR (B) neonates are shown. Representative 2-D gel images of UC plasma from AGA (A) and IUGR.

Confocal fluorescence microscopical images of 3-nitrotyrosine-positive blood vessels from a patient with mitochondrial disease. Confocal fluorescence microscopical.

IHC staining of various cystic structures

Schematic summarizing the various functions and features of MASH Suite Pro. Schematic summarizing the various functions and features of MASH Suite Pro.

Protein microarrays for validation of antibodies.

Two-dimensional gel electrophoresis of CyDye-labeled human sperm proteomes (DIGE) and identification of diabetes- and obesity-associated sperm proteins.

A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. A, Western blot analysis of fetuin-A.

Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. Testing.

Altered pathways in prostate cancer.

Analysis of whole cell lysates by Western blotting and 2D gel electrophoresis.A, cells overexpressing GFP fusion proteins and control cells were cultured.

Interaction networks of the regulated phosphoproteins.

The PPAR-α agonist GW7647 increases the levels of apoA-I in the retina and the fibrous sclera. The PPAR-α agonist GW7647 increases the levels of apoA-I.

IEF 2D PAGE of whole protein extracts from breast apocrine macrocysts

LC-MS/MS analyses of synthetic peptides with SUMO1 and SUMO3 remnant chains using ETD, CID, and HCD activation modes. LC-MS/MS analyses of synthetic peptides.

Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum. Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum.A,

Spotting pattern of Arabidopsis protein microarrays.

Distribution of the phosphoproteins based on GO analysis, including biological process (Left) and cellular component (Right). Distribution of the phosphoproteins.

Examples of protein that display profiles corresponding to GO/GROW and STOP signals.A, example of STOP profile: normalized spot volume profile of apoA-I.

Extraction of proteins from MALDI IMS slides.

Differential expression of apoA-I and Vimentin on 2D gels

K-Means clustering of protein and mRNA expression patterns after PPAR agonists treatments. k-Means clustering of protein and mRNA expression patterns after.

Number of genes/antibodies included in the database.

Immuno-MS results from antibodies toward 20 different target proteins in HeLa cell lysates. Immuno-MS results from antibodies toward 20 different target.

Biochemical characterization of the protein phosphatases Saci-PTP.

Preferential conjugation of proteins to SUMO-1 or SUMO-2

Changes in mRNA levels do not correlate with changes in protein levels in upf1Δ and xrn1Δ cells. Changes in mRNA levels do not correlate with changes in.

Classification of the 1458 identified proteins into molecular functions. Classification of the 1458 identified proteins into molecular functions. The pie.

Separation of colonospheres from differentiated tumor cells by cluster analysis. Separation of colonospheres from differentiated tumor cells by cluster.

Comparison of HCT-8 cell line proteome under apoptotic conditions in response to 10 mm Gln treatment Spot numbers with corresponding Swiss-Prot database.

2-D gel images visualized by Coomassie Brilliant Blue staining representing total proteins extracted from HCT-8 under apoptotic conditions in 2 mm Gln.

Enlargements of 2-D gels visualized by Coomassie Brilliant Blue staining. Enlargements of 2-D gels visualized by Coomassie Brilliant Blue staining. In.

Immunoblot analysis of apoptotic HCT-8 cell extracts separated by SDS-PAGE. Immunoblot analysis of apoptotic HCT-8 cell extracts separated by SDS-PAGE.

Proteomic analysis of invasive TCCs

Expression of σ in SCCs Expression of σ in SCCs. Shown is a magnified section of a representative 2D PAGE gel run with a lysate from an SCC.

Antibody specificity ascertained by 2D-PAGE Western immunoblotting (IEF) of total cellular protein extracts from the RT4 human bladder cancer cell line.

Western blotting analysis of purified cytoplasmic membranes.

Proteomics analysis of NaPi-IIa C terminus binding to PDZ proteins.

SiRNA knockdown of dynein IC2-C recovered the inhibition of neurite outgrowth in NF1-KD PC12 cells. siRNA knockdown of dynein IC2-C recovered the inhibition.

GeneGoTM-based signaling pathway annotations of proteins identified in CD56+ NK cell subsets. GeneGoTM-based signaling pathway annotations of proteins.

Changes in protein expression during distinct stages of NK cell differentiation. Changes in protein expression during distinct stages of NK cell differentiation.

The average median S.D. and PEV reduction after applying different normalization methods compared with raw data. The average median S.D. and PEV reduction.

Model of the change in receptor structure on engagement of the ligand IFN-γ. Model of the change in receptor structure on engagement of the ligand IFN-γ.

Presentation transcript:

Two-dimensional gel silver staining and two-dimensional immunoblotting using antibody to 3-nitrotyrosine. Two-dimensional gel silver staining and two-dimensional immunoblotting using antibody to 3-nitrotyrosine. Immunoprecipition of vimentin, desmin, ATP synthase β-chain subunit, protein disulfide-isomerase A3, voltage-dependent anion channel 1 and MnSOD and immunoblotting using antibody to 3-nitrotyrosine. A-Matching immunoreactive spots specifically present in MD patients are squared and numbered in a representative two-dimensional silver-stained gel and in the corresponding anti-3-nitrotyrosine two-dimensional immunoblotting of a patient (P) and a control (C) before and following reduction (AR). The immunopositive spots detected following the reduction were not considered for further analysis. The corresponding proteins were identified as reported in Table III. B-Anti-3-nitrotyrosine two-dimensional immunoblotting following immunoprecipitation of vimentin, desmin, ATP synthase β-chain subunit, protein disulfide-isomerase A3, voltage-dependent anion channel 1, and MnSOD shows expected spots corresponding to proteins. Gaetano Vattemi et al. Mol Cell Proteomics 2011;10:M110.002964 © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.