Common 677C→T mutation of the 5,10-methylenetetrahydrofolate reductase gene affects follicular estradiol synthesis  Stephanie Hecht, Roman Pavlik, Peter Lohse, M.D., Ulrich Noss, M.D., Klaus Friese, M.D., Christian J. Thaler, M.D.  Fertility and Sterility  Volume 91, Issue 1, Pages 56-61 (January 2009) DOI: 10.1016/j.fertnstert.2007.11.011 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Estradiol production of cultured human granulosa cells with regard to the MTHFR 677C→T genotype. Box plots show median, interquartile range, outliers (black dots), and extreme cases of individual variables within the 5th and 95th percentiles. Experiments (n = 25 for C/C patients; n = 36 for C/T patients; n = 5 for T/T patients) were performed after 72 hours. Human granulosa cells were stimulated with either 80 IU/L r-FSH (B) or 80 IU/L r-LH (C) for 48 hours or were sham stimulated (A). Androstenedione (10−6 mol/L) was added to all cultures as a substrate. Fertility and Sterility 2009 91, 56-61DOI: (10.1016/j.fertnstert.2007.11.011) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Calculated E2–protein ratio of individual follicular fluids with regard to the MTHFR 677C→T genotype. Box plots show median, interquartile range, outliers (black dots), and extreme cases of individual variables within the 5th and 95th percentiles. Fertility and Sterility 2009 91, 56-61DOI: (10.1016/j.fertnstert.2007.11.011) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions