Discovery and development of the N-terminal procollagen type II (NPII) biomarker: a tool for measuring collagen type II synthesis O.V. Nemirovskiy, Ph.D.,

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Presentation transcript:

Discovery and development of the N-terminal procollagen type II (NPII) biomarker: a tool for measuring collagen type II synthesis O.V. Nemirovskiy, Ph.D., T. Sunyer, Ph.D., P. Aggarwal, Ph.D., M. Abrams, M.S., M.P. Hellio Le Graverand, Ph.D., W.R. Mathews, Ph.D. Osteoarthritis and Cartilage Volume 16, Issue 12, Pages 1494-1500 (December 2008) DOI: 10.1016/j.joca.2008.04.021 Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 LC-MS spectra of the NPII fragments. Each spectrum represents one peak in the reverse phase-high performance liquid chromatogram (RP-HPLC) obtained by immunoaffinity pull-down of the procollagen II peptides from (A) HAC, (B) plasma, and (C) urine samples. Different charge states for various forms of the QDVRQPGPKGQKGEPGDIKD peptide with up to four hexose moieties were detected. (D) An MS/MS spectrum of the QDVRQPGP(aK)GQ(HyK)GEPGDIKD with three Hex moieties, m/z 884.03 for the triple charged parent ion. Both N- and C-terminal fragment ions were observed allowing for manual sequence identification. In addition, a facile loss of sugar moieties was observed. Osteoarthritis and Cartilage 2008 16, 1494-1500DOI: (10.1016/j.joca.2008.04.021) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Cross-sectional evaluation of the NPII levels in the plasma of reference control and OA patients. Statistically significant (***P<0.0001) difference was observed between symptomatic OA patients with radiographic OA in the hip(s) and/or knee(s) and a reference control group consisting of healthy volunteers without symptoms and radiographic OA in the hip(s) and/or knee(s); a subset of the first group (n=17) was presented with radiographic OA in the smaller joints such as hand(s) and/or spine. Osteoarthritis and Cartilage 2008 16, 1494-1500DOI: (10.1016/j.joca.2008.04.021) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions